L-Glutamine, 200 mM solution, tends to precipitate in solution when chilled. After thawing, it will be an opalescent solution. If allowed to settle, there can be some crystalline insolubles at the bottom of the bottle.The product should be warmed to 37°C for complete solubilization. To limit the number of times the product needs to be warmed to 37°C, the product can be divided into working aliquots which are stored at -20°C and used as needed. This will expose the product to the minimal amount of warming cycles.
推薦產品
等級
Hybri-Max™
品質等級
無菌
sterile-filtered
化驗
≥99% (TLC)
形狀
solution
技術
cell culture | hybridoma: suitable
雜質
endotoxin, tested
顏色
clear colorless
運輸包裝
dry ice
儲存溫度
−20°C
SMILES 字串
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI 密鑰
ZDXPYRJPNDTMRX-VKHMYHEASA-N
基因資訊
rat ... Ggt1(116568)
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一般說明
L-谷氨酰胺是一种必需氨基酸，作为细胞培养过程中主要的能量来源而成为培养基的重要组分。L-谷氨酰胺在干粉和冷冻溶液中非常稳定。然而，在液体培养基或储备溶液中，L-谷氨酰胺的降解速度相对更快。为获得最佳细胞性能，通常需要在使用培养基前补充L-谷氨酰胺。
應用
L-谷氨酰胺溶液已用作罗斯威尔公园纪念研究所（RPMI）-1640培养基的补充剂，以培养人白血病单核细胞系用于细胞内分析和脾细胞用于CD4+ T细胞的体外激活。它还用作杂交瘤培养基的补充剂，用于抗原结合和杂交瘤克隆的初级筛选。
準備報告
使用细胞培养级水进行制备。
法律資訊
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
應用
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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文章
Importance and uses of glutamine in hybridoma and mammalian cell culture
谷氨酰胺在雜交瘤和哺乳動物細胞培養中的重要性和用途
Chromatograms
application for HPLC-
Why is there a precipitate in the 200 mM L-glutamine solution, products G7513 and G2150, after thawing at room temperature?
1 answer-
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Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
1 answer-
Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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