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F2442

Sigma-Aldrich

胎牛血清

Fetal Bovine Serum
1 of 1 reviewers received a sample product or took part in a promotion

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

同義詞:

FBS, FCS, 血清

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About This Item

MDL號碼:
MFCD00132239
分類程式碼代碼:
12352207
eCl@ss:
32160409
NACRES:
NA.71
生物源:
bovine fetus
來源:
USA origin
無菌:
sterile-filtered
運輸包裝:
dry ice
暫時無法取得訂價和供貨情況

生物源

bovine fetus

品質等級

500

無菌

sterile-filtered

產品線

FBS Classic

成份

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

來源

USA origin

技術

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

雜質

≤10 EU/mL endotoxin

運輸包裝

dry ice

儲存溫度

−20°C

尋找類似的產品？ 前往 產品比較指南

一般說明

胎牛血清（FBS）是一种由低、高分子量生物分子组成的复杂混合物，具有最佳的生长促进和抑制功能。FBS中含有如生长因子、蛋白质、维生素、微量元素、激素等多种分子。这些分子对于细胞的生长和维持至关重要。它是细胞培养基中的一种常见补剂。[1][2]FBS来自于小牛血。它不含有纤维蛋白和凝血因子。FBS的热灭活可阻断补体系统。[3]来源于凝结血的FBS是真核细胞，尤其是哺乳动物细胞培养中最广泛使用的未确定补充剂。[4][5]它通常与在1950年代和1960年代开发的经典培养基一起使用。这些包括多种常用培养基，如Minimum Essential培养基（MEM）、[6]Dulbecco′s改良Eagles培养基（DME，DMEM）、Iscove′s改良Eagles培养基（IMDM）、[7]M199[8]以及Roswell Park Memorial Institute培养基RPMI-1640。[6]

應用

胎牛血清还可作为组分用于：
  • 血管周围巨噬细胞样黑素细胞（PVM/M）生长培养基[9]
  • 合成输卵管液（SOF）[10]
  • Eagle′s minimum essential培养基（EMEM）[11]
  • neurobasal培养基[12]
  • Hyclone McCoys 5 A培养基[13]
  • Hanks培养基[14]

生化／生理作用

FBS仍然是一种常用的培养基补充剂，因为它在细胞培养中提供了广泛的功能。FBS提供营养、生长和附着因子，并通过难以在无血清培养基（SFM）系统中复制的机制保护细胞免受氧化损伤和细胞凋亡。

分析報告

  • 内毒素和血红蛋白测试
  • 检测了是否存在细菌、病毒和支原体
  • 在无菌条件下用0.1微米膜过滤三次

其他說明

对是否存在细菌、病毒、支原体、内毒素以及血红蛋白进行了检测。在无菌条件下用0.1微米膜过滤三次。

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
19G057-A
24B128
0000321240
23A316
23A317

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
Mosa FM, et al.
Ife Journal of Science, 4B, 3088-3093 (2015)
Inhibiting tyrosine phosphorylation of protein kinase Cd (PKCd) protects the salivary gland from radiation damage.
Wie SM et al.
The Journal of Biological Chemistry, 289, 10900-10900 (2014)
MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.
Grigoryev SA et al.
The Journal of Biological Chemistry, 274, 5626-5626 (1999)
Cunzhi Yu et al.
Frontiers in pharmacology, 8, 832-832 (2017-12-07)
Low solubility, tissue accumulation, and toxicity are chief obstacles to developing triptolide derivatives, so a better understanding of the pharmacokinetics and toxicity of triptolide derivatives will help with these limitations. To address this, we studied pharmacokinetics and toxicity of (5R)-5-hydroxytriptolide
Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)
查看所有相關論文

文章

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High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

細胞培養中的鈣

鈣是一種離子穩定的二價陽離子，在細胞培養中具有重要的有益和有毒特性。它是各種細胞培養基的成分之一。

條款

使用包裝混合質粒製備慢病毒轉導粒子

在此包裝系統中，與 VSV-G 的偽型擴大了與病毒相關的病毒滋養性。然後，慢病毒顆粒可將序列傳遞至各種細胞類型，包括原代細胞和非分裂細胞。

Lentiviral Transduction Protocol

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慢病毒轉導協議

如何進行 MISSION shRNA 慢病毒顆粒的慢病毒轉導，以達到長期穩定的沉默和表型改變的詳細程序。

相關內容

Evaluating of Stericup® Quick Release Filtration Devices for Fetal Bovine Serum (FBS) in Cell Culture

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Protocol Guide: Generation of Apical-Out Gastrointestinal Organoids

Step-by-step protocol for generating apical-out human gut organoids for microbiome, ADME/Tox, viral and gastrointestinal related disease research. See the complete organoid culture protocol.

協議指南：生成根尖向外的胃腸有機體

用於微生物組、ADME/毒性、病毒和胃腸道相關疾病研究的分步式人體腸道器官模型製作方案。請參閱完整的有機體培養方案。

符合标准的FBS胎牛血清。

探索我们广泛的胎牛血清(FBS)产品组合并根据您的实验室需求和预算选择合适的产品。了解全球事件如何影响FBS价格。

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Questions

1–10 of 15 Questions  

  1. How to heat inactivate this serum Fetal Bovine Serum USA origin,(Cat Log - F2442)

    1 answer

    1. Heat inactivation occurs when serum is heated to 56°C for a period of 30 minutes.

      Here's a protocol for heat inactivation:
      1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
      2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
      3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
      4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.

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  2. is this Heat Inactivate FBS?

    1 answer

    1. This product has not been heat inactivated.

      Helpful?

  3. can F2442 FBS be used for culturing peritoneal macrophages? what is the suitable one?

    1 answer

    1. This product is tested for suitability in mammalian cell culture. While it is not specifically tested on macrophages, it is subject to stringent specifications, including low endotoxin, and should perform well. Please see the link below to review a sample Certificate of Analysis:
      https://www.sigmaaldrich.com/coa/SIGMA/F2442/23A317

      Helpful?

  4. Can this f2442 fbs be used for culture expansion of mesenchymal stem cells? Please confirm Thanks

    1 answer

    1. Fetal Bovine Serum (Cat. no.: F2442) is suitable for use in mammalian cell culture. However, it is not tested specifically in mesenchymal stem cell culture. There is some evidence in the literature that this product would be suitable. See the link below for a relevant reference:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545441/

      Helpful?

  5. What is the difference between Fetal Bovine Serum F2442 and 12103C?

    1 answer

    1. Product No. 12103C meets most of the specifications of F2442.  Both products are from the same raw material suppliers and undergo the same stringent testing prior to release. However, the F2442 has been use-tested in hybridoma culture, whereas 12103C has not.

      Helpful?

  6. There is a precipitate in my serum.  Is my serum contaminated?

    1 answer

    1. Precipitates in serum can often appear as large round bodies, even as doublets per microscopic exam. This can be mistaken as a yeast contamination. Brownian motion may be mistaken for motility. These are lipid aggregates that will be readily dissolved when exposed to lipase. Also, these particles will not gram stain or show any growth in culture on aerobic, anaerobic, or fungal media and culture conditions. The performance of the material is not effected. Centrifugation or filtration of small volumes will remove these particles.

      Helpful?

  7. What is the cloning assay performed on Fetal Bovine Serum, Product F2442?

    1 answer

    1. The cloning efficiency assay is performed on each lot of FBS to verify that each lot will support clonal growth of myeloma cells and/or fusion products. The assay is performed using 5% and 10% concentrations of the FBS.

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  8. How is Fetal Bovine Serum, Product F2442, cell culture tested?

    1 answer

    1. Biological performance is assessed using Sigma cell culture medium supplemented with the serum and tested at a final concentration of 10%. Seeding efficiency, doubling time, and final cell density are measured on appropriate cell lines. Growth studies are carried through two subculture generations and cell growth is plotted as a logarithmic function of time in culture. During the testing period, cultures are examined microscopically for atypical morphology and evidence of cytotoxicity. Hybridoma -tested serum has been shown to support mouse myeloma cells and hybridoma cells in culture.

      Helpful?

  9. Is Fetal Bovine Serum, Product F2442, available as a charcoal stripped, de-lipidated serum?

    1 answer

    1. We offer Product No. F6765 (Fetal Bovine Serum, charcoal stripped). The charcoal stripping decreases the levels of hormones; it may also decrease the levels of lipids. If further reduction of lipid levels is required, you may need to treat the serum with fumed silica (e.g. Product No. S5130). Use 20 grams of S5130 per Liter of serum (not plasma). It is mixed for 3-4 hours, then centrifuged in 1 L containers for about 15 minutes at 2000 xg. The supernatant can then be decanted, and filtered if desired. NOTE: Silica is extremely fluffy, and care must be taken not to inhale its dust. Allow for about 15% loss of the serum due to adsorption.

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  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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Reviews

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    5 out of 5 stars.

    최고의 갓성비 FBS!

    정기배송으로 진행시 가격도 50만원으로 저렴하고 정해진 날짜에 와서 좋아요!
    DMSO같이 많이 쓰는 제품도 media와 같이 묶어서 사용하면 더 저렴해지더라구요 ㅎ

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