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Merck
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文件

D8062

Sigma-Aldrich

DMEM/F-12

With ʟ-glutamine, sodium bicarbonate and sodium pyruvate, sterile liquid, suitable for cell culture

同義詞:

DME/F-12, 1:1混合

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About This Item

MDL號碼:
MFCD00217417
分類程式碼代碼:
12352207
NACRES:
NA.75

product name

杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L

運輸包裝

ambient

儲存溫度

2-8°C

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相關類別

一般說明

在成分确定的培养基研究中，通常用成分确定的混合物（包括营养素、生长因子和激素）替代血清添加到培养基中。常用的组合是Dulbecco′s改良Eagle培养基(DME)与Ham′s F-12营养混合物（1:1混合）。为了补偿去血清后的缓冲能力损失，可在配方中添加15 mM终浓度的HEPES缓冲液，帮助研究人员优化缓冲体系。

應用

Dulbecco′s改良Eagle培养基/Ham F-12营养混合物已用于：
  • 维持人肺和结直肠癌细胞系生存，进行次级代谢产物的生物学评估
  • 培养人肾-2细胞(HK-2)并作为检测培养基组分，用于溶血磷脂酰胆碱(LPC)处理
  • 小鼠鳞状细胞癌(SCCVII)肿瘤细胞培养

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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