跳轉至內容
Merck
全部照片(1)

重要文件

D6421

Sigma-Aldrich

DMEM/F-12

With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

同義詞:

DME/F-12, 1:1混合

登入查看組織和合約定價


About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75
暫時無法取得訂價和供貨情況

產品名稱

杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

sodium pyruvate: 0.055 g/L
L-glutamine: no
phenol red: yes
NaHCO3: yes
HEPES: 15 mM
glucose: 3.15 g/L

運輸包裝

ambient

儲存溫度

2-8°C

尋找類似的產品? 前往 產品比較指南

一般說明

由精确测量、化学成分确定的混合物构成的基础培养基,其是一种复合培养基。DMEM是一种补充有血清的培养基,有时还包含胰蛋白胨磷酸盐肉汤(TPB)成分。DMEM/F-12是一种成分完全确定的合成培养基,其不含蛋白质成分。[1]

應用

DMEM:F12是DMEM和Ham's F12培养基的50:50混合物,其已被证明可用于广泛的细胞培养应用中,特别是当需要补充胎牛血清(FBS)时。 Ham′s Nutrient Mixture F12最初是为中国仓鼠卵巢(CHO)细胞、肺细胞和小鼠L细胞的无血清克隆生长而开发的。它经常与透析的血清、激素、硒和其他补充剂一起用于无血清培养。它是支持啮齿动物来源细胞生长的首选培养基,特别是兔和大鼠,其已被证明是骨髓瘤和杂交瘤细胞的优良克隆培养基。
适合在低血清条件下生长多种细胞类型。
杜氏′改良Eagle′s 培养基/营养混合物F-12 Ham可用于细胞培养&增殖检测。[2][3][4][5]它还可用于骨髓衍生的巨噬细胞的培养。[6]

重構

以0.365g/L补充 L-谷氨酰胺。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

未看到正確版本?

如果您需要一個特定的版本,您可以透過批號來尋找特定憑證。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Yohei Ueda et al.
Scientific reports, 9(1), 8547-8547 (2019-06-14)
Growth retardation is an important side effect of glucocorticoid (GC)-based drugs, which are widely used in various preparations to treat many pediatric diseases. We investigated the therapeutic effect of exogenous CNP-53, a stable molecular form of intrinsic CNP, on a
E Zweygarth et al.
The Onderstepoort journal of veterinary research, 70(2), 165-168 (2003-09-12)
The Welgevonden stock of Ehrlichia ruminantium, aetiological agent of heartwater, was propagated in baby hamster kidney (BHK) cells, Chinese hamster ovary (CHO-K1) cells and Madin Darby bovine kidney (MDBK) cells. The cultures required supplementation of the medium with cycloheximide for
Kouichi Hasegawa et al.
Stem cells translational medicine, 1(1), 18-28 (2012-12-01)
An optimal culture system for human pluripotent stem cells should be fully defined and free of animal components. To date, most xeno-free culture systems require human feeder cells and/or highly complicated culture media that contain activators of the fibroblast growth
Samir A Saidi et al.
Molecular cancer, 5, 13-13 (2006-03-30)
Fenofibrate, an agonist of PPAR-alpha, in doses above 25 microM, inhibits proliferation and induces apoptosis in Ishikawa endometrial cancer cells. We show that these effects are potentiated by retinoic acid, an agonist of the retinoid-X-receptor. DNA content analysis shows that
Xi Chen et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(21), 9202-9213 (2013-05-24)
Progranulin (PGRN) is a secreted glycoprotein expressed in neurons and glia that is implicated in neuronal survival on the basis that mutations in the GRN gene causing haploinsufficiency result in a familial form of frontotemporal dementia (FTD). Recently, a direct

文章

Nutrient Mixture F-12 Ham Formulation

營養混合物 F-12 火腿配方

條款

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

人類誘導多能幹細胞 (iPSC) 的分步式幹細胞培養方案,包括 ips 細胞解凍、擴大、冷凍和特性分析。

Questions

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務