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D8537

Sigma-Aldrich

杜氏磷酸盐缓冲盐水

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

同義詞:

DPBS

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About This Item

MDL號碼:
MFCD00131855
分類程式碼代碼:
12352207
eCl@ss:
32129211
NACRES:
NA.75

product name

杜氏磷酸盐缓冲盐水, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

品質等級

300

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

glucose: no (Dextro)
sodium pyruvate: no

運輸包裝

ambient

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相關類別

一般說明

杜氏磷酸盐缓冲盐水（D-PBS）是一种用于处理和培养哺乳动物细胞的平衡盐溶液（BSS）。DPBS缓冲液可用于冲洗、洗涤并稀释哺乳动物细胞，同时维持渗透平衡。它提供正常细胞代谢所必需的水和无机离子。DPBS的钙和镁有助于细胞的结合和成团。不含这些离子的DPBS可用于悬浮后细胞的洗涤和润洗。磷酸缓冲体系将pH维持在生理性范围内。

應用

Dulbecco′s磷酸盐缓冲液已用于：

  • 稀释新鲜红细胞用于研究红细胞聚集物的捕获。
  • 清洗微量滴定板的微孔
  • 在裂解缓冲液中重悬细胞以前清洗细胞进行DNA分离
  • 作为胚胎保存培养基的成分用于胚胎的玻璃化。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

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產品號碼
描述
訂價

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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Elliptical optical tweezers for trapping a red blood cell aggregate
Kauppila AJ
Photonics Letters of Poland, 3(3), 95-97 (2011)
Arturo Levican et al.
Applied and environmental microbiology, 79(16), 4951-4957 (2013-06-19)
The genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri, A. cryaerophilus, and A. skirrowii, as these have been associated with human cases of diarrhea, the probable transmission routes
Casper Hyttel Clausen et al.
Sensors (Basel, Switzerland), 18(10) (2018-10-20)
Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential
Cameron Goertzen et al.
Biological procedures online, 20, 20-20 (2018-10-26)
Invadopodia are actin-rich, proteolytic structures that enable cancer cell to invade into the surrounding tissues. Several in vitro invasion assays have been used in the literature ranging from directional quantitative assays to complex three-dimensional (3D) analyses. One of the main
Wishrawana S Ratnayake et al.
International journal of oncology, 51(5), 1370-1382 (2017-10-20)
Atypical protein kinase Cs (aPKC) are involved in cell cycle progression, tumorigenesis, cell survival and migration in many cancers. We believe that aPKCs play an important role in cell motility of melanoma by regulating cell signaling pathways and inducing epithelial
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Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.

黏附細胞系的亞培養

使用胰蛋白酶 EDTA 傳代和分裂黏附細胞株的細胞培養規程。免費下載 ECACC 手冊。

Cryopreservation of Cell Lines

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

細胞株的冷凍保存

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