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品質等級
100
200
形狀
liquid
用途
200 uses (0.1 mg size)
40 uses (20 μg size)
適合性
suitable for electrophoresis (DNA)
儲存溫度
−20°C
相關類別
一般說明
Sigma′s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
應用
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
pUC18 DNA Hae III Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen.[1]
成分
Sigma′s pUC18 DNA Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
其他說明
Contains 11 fragments, 11–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5%.
準備報告
Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction.
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
客戶也查看了
Development of competitive PCR for detection of Butyrivibrio fibrisolvens in the rumen
Mrazek J and Kopecny J
Folia Microbiologica, 46(1), 63-65 (2001)
文章
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
用於凝膠電泳的標記有助於確定 DNA、PCR 片段和 RNA 的大小，與常見的核酸染色劑染色效果良好。
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
透過瓊脂糖或聚丙烯酰胺凝膠上的電泳，選擇適當的標記和梯子進行核酸大小測定。
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