The AMPD1 product, DNase I component D5307, is not tested for protein content, so it is not possible to determine the activity units per mg of protein. This variability in active protein content within a preparation makes it more accurate to sell the product based on its unit activity. If the protocol specifies a certain number of milligrams of DNaseI, it is advisable to determine the specific activity of the enzyme used in the procedure. Alternatively, a titration analysis may be necessary to determine the appropriate amount to use.
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一般說明
当前没有任何 RNA 分离程序可以 100% 去除 DNA。 许多商业DNase I 制剂受到了残留RNase污染， 这种RNase污染会在逆转录以前破坏或分解有价值的RNA样品。实验室比较表明，Sigma′的扩增级 DNase I 的RNase活性低于几家领先的分子生物学产品供应商产品。
脱氧核糖核酸酶I（DNase I）是一种从牛胰腺中分离的内切核酸酶，可将双链和单链DNA消化成寡核苷酸和单核苷酸。 扩增级 DNase I 已纯化去除RNase活性，适于在 RT-PCR（逆转录酶 - 聚合酶链反应）等敏感应用之前去除 RNA 制剂中的DNA。
DNase I可将双链和单链DNA酶解成寡核苷酸和单核苷酸。采用提供的反应缓冲液，在室温下消化 15 分钟，可去除 RNA 制剂中的 DNA。然后随同终止液加热，使DNase I失活。加热还会使RNA中的发夹变性，可直接用于逆转录。
当前没有任何 RNA 分离程序可以 100% 去除 DNA。 许多商业DNase I 制剂受到了残留RNase污染。 这种RNase污染会在逆转录以前破坏或分解有价值的RNA样品。实验室比较表明，Sigma′的扩增级 DNase I 的RNase活性低于几家领先的分子生物学产品供应商产品。
DNase I可将双链和单链DNA酶解成寡核苷酸和单核苷酸。采用提供的反应缓冲液，在室温下消化 15 分钟，可去除 RNA 制剂中的 DNA。然后随同终止液加热，使DNase I失活。加热还会使RNA中的发夹变性，可直接用于逆转录。
当前没有任何 RNA 分离程序可以 100% 去除 DNA。 许多商业DNase I 制剂受到了残留RNase污染。 这种RNase污染会在逆转录以前破坏或分解有价值的RNA样品。实验室比较表明，Sigma′的扩增级 DNase I 的RNase活性低于几家领先的分子生物学产品供应商产品。
應用
特點和優勢
- 适用于去除 RNA 中的 DNA
- 超低RNase活性
- 经过优化的10×反应缓冲液和终止液，可使DNase I完全失活
適合性
适用于去除 RNA 制剂中的 DNA。
單位定義
在 37 °C 下，一个单位可在 10 min 内将 1μg 质粒DNA 消化为寡核苷酸。
法律資訊
购买本产品附带有限的许可，仅可用于研究用途聚合酶链式反应 (PCR) 过程，且应配合热循环仪使用。该热循环仪在 PCR 过程的自动执行中的使用由预先许可费涵盖，该费用通过向 Applied Biosystems 支付或购买的方式（即授权的热循环仪）支付。
抑制劑
相關產品
產品號碼
描述
訂價
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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文章
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
使用 MULTI-seq 脂質修飾寡聚體、PCR Assays 和 Sequencing 應用的協議和故障排除指南。
條款
SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法，
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
將 RNA 反轉錄至 DNA 的方法。使用預混合試劑，其中包含逆轉錄酶、dNTPs、引物、RNase 抑制劑和緩衝液。快速產生 cDNA。
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
相關內容
The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
聚合酶連鎖反應 (PCR) 是一種擴增核酸分子的技術，常用於 RT-PCR、熱起始 PCR、終點 PCR 等多種應用。
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How can I convert 'units' to mass when adding DNaseI to a buffer solution?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What do I do if my DNA is not completely digested after 15 minute digestion at room temperature when using Product AMPD1, DNase I?
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If further digestion is required, incubation can be performed for 30 minutes at 37°C.
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What is the concentration of the DNAse in Product AMPD1, DNase I?
1 answer-
The DNAse is provided at 1 unit/uL in 1 mL total volume.
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