This product is assigned a minimum activity specification of 2000 units per milligram protein and a minimum protein specification of 80%. At the minimum value for each specification, the 150,000 unit package size would contain 93.75 mg of powder using the following calculation:
Units/(activity x %protein) = mass or 150,000/(2000 x 0.80) = 93.75 mg
The activity and protein content are lot-specific and reported in the product Certificate of Analysis. As the activity increases the mass per package size decreases. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/d5025#product-documentation
推薦產品
生物源
bovine pancreas
種類
Type IV
形狀
lyophilized powder
比活性
≥2,000 Kunitz units/mg protein
分子量
~31 kDa
純化經由
chromatography
成份
Protein, ≥80%
技術
DNA purification: suitable
溶解度
0.15 M NaCl: soluble 5.0 mg/mL, clear, colorless
適合性
suitable for molecular biology
應用
diagnostic assay manufacturing
diagnostic assay manufacturing
異物活動
Chymotrypsin ≤0.5%
Protease ≤0.05%
RNase ≤0.02%
運輸包裝
wet ice
儲存溫度
−20°C
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應用
DNAse I可用于切开DNA，作为将标记碱基插入DNA的第一步。来自sigma的该酶已用于处理大鼠的脑组织。该研究表明星形胶质细胞的轴突生长不受少突胶质细胞抑制。[1]在另一项研究中，大肠杆菌的解冻固定样本被来自Sigma的DNAseI以及其他酶进行消化。消化是在进行通透化处理和核酸染色前完成的。[2]
来自牛胰腺的脱氧核糖核酸酶I已用于研究枯草芽孢杆菌中核酸酶的二维酶谱分析。来自牛胰腺的脱氧核糖核酸酶I还用于研究小沟和大沟结合药物和嵌入剂对小沟结合蛋白RecA和脱氧核糖核酸酶I的DNA结合的影响。
用于从蛋白质样品中除去 DNA。
生化／生理作用
DNase I是一种内切核酸酶，可作用于与嘧啶相邻的磷酸二酯键以产生具有末端5′-磷酸的聚核苷酸。当Mg2+存在时，DNAse I可独立切割DNA的每条链且切割位点是随机的。当Mn2+存在时，两条DNA链都几乎是在同样的位置被切割。[3]最适pH在7-8之间。二价阳离子如Mn2+, Ca2+, Co2+以及Zn2+都是酶的激活剂。[4]5 mM浓度的Ca2+可稳定酶免收蛋白水解酶的消化。来自牛胰腺的DNAse I由四种色谱可区分的组分A，B，C和D组成，它们的摩尔比为4:1:1。仅有少量的D被发现。[5]2-巯基乙醇、螯合剂、十二烷基硫酸钠（SDS）[6]和肌动蛋白[7]都已知可抑制酶的活性。
單位定義
一Kunitz单位的酶在以I型或III型DNA作为底物时，在 pH 5.0，37°C的条件下在每mL中每分钟可引起0.001的A260。
外觀
含氯化钙的冻干粉
準備報告
溶液0.15 M NaCl的10 mg/mL DNAse I溶液分装保存在−20 °C一周后可能会丢失<10%的活性。同样的溶液保存在2-8 °C则可能丢失约20%的活性。当在pH 5和7之间时，它可在60 °C维持活性最长达五小时，并在68 °C <10分钟内丢失活性。它在乙酸缓冲液（pH 5.0）和tris缓冲液((pH 7.2)，1 mg/mL浓度)中以6%/小时的速率丢失活性。
分析報告
蛋白由双缩脲法测定。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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條款
建立脱氧核糖核酸酶I酶促测定的标准化操作流程。
To standardize a procedure for the enzymatic assay of Deoxyribonuclease I.
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How many milligrams are in one vial of D5025-150KU?
1 answer-
Helpful?
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Good afternoon, if the stock concentration of 10mg/ml may loose 10% of its activity after one week, I was wondering if it is okey regarding stability to keep 500ug/ml aliquots at -20ºC for several weeks.
1 answer-
As cited in the product listing and datasheet, this product in solution shows an approximate loss of activity of 10% when stored in aliquots at −20 °C for 1 week. Studies at lower dilutions have not been performed. Typically, solutions stored at higher concentrations are the most stable.
Helpful?
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Is Kunitz unit same as unit here? I have seen some papers and they have U/ml for DNase. How can i conver it?
1 answer-
Product D5025, Deoxyribonuclease I is given in Kunitz units. This product is a powder. When reconstituting the product with a solution, for example 15,000 Kunitz units in 1 ml of 0.15 M NaCl the end-user will have 15,000 Kunitz units/ml.
Helpful?
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How do Deoxyribonuclease I (DNase I), products D5025 and D4527 differ from each other?
1 answer-
Both of these products have the same specific activity (>2000 units/mg protein) for Dnase I. The enzyme impurity levels for each, however, are different. D5025 has the following impurity specification: Chymotrypsin (<0.5%), Protease (<0.05%), and RNase (<0.02%). The impurity specifications for D4527 are: Chymotrypsin (<0.01%), Protease (<0.005%), and RNase (<0.01%).
Helpful?
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Are stock solutions of Product D5025, Deoxyribonuclease I (DNase I), stable?
1 answer-
Solutions of DNAse I (10 mg/ml) in 0.15 M NaCl may lose 10% of its activity when stored for a week in aliquots at -20°C. The same solutions stored in aliquots at 2 - 8°C. lose approximately 20% activity.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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How can I convert units of Product D5025, Deoxyribonuclease I (DNase I), to mass?
1 answer-
The mg of solid for each unit package size will be indicated on the label of the package. For a particular lot, you can calculate the specific activity in units per mg solid by multiplying the specific activity in units per mg protein by the percent protein divided by 100.
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How should I prepare a solution of Product D5025, DNAse I?
1 answer-
This enzyme can be reconstituted in 0.15 M NaCl at a concentration of 10 mg/ml.
Helpful?
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What concentration of Product D5025, Deoxyribonuclease I (DNase I), is required to remove DNA from solutions?
1 answer-
DNA can be removed from preparations by incubation with 20 - 50 ug DNAse I in the presence of 50 mM Tris-HCl buffer, pH 7.5, 10 mM magnesium chloride, at 37°C. for 60 min.
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