We do not test this product for IF on cells. However, it has been used in this application. You can use a secondary antibody like Product No. Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat.
A2547
单克隆抗-肌动蛋白，α-平滑肌
clone 1A4, ascites fluid
同義詞:
α平滑肌肌动蛋白抗体 - 单克隆抗Actin, α-平滑肌, α平滑肌肌动蛋白抗体Sigma, 抗α平滑肌肌动蛋白抗体, SMA
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About This Item
分類程式碼代碼:
12352203
NACRES:
NA.41
共軛:
unconjugated
application:
IF
IHC (f)
IHC (p)
WB
IHC (f)
IHC (p)
WB
無性繁殖:
1A4, monoclonal
物種活性:
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
citations:
2586
技術:
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells
immunohistochemistry (frozen sections): suitable using smooth muscle cells
indirect immunofluorescence: 1:400 using blood vessels in sections of human appendix
western blot: suitable using smooth muscle cells
immunohistochemistry (frozen sections): suitable using smooth muscle cells
indirect immunofluorescence: 1:400 using blood vessels in sections of human appendix
western blot: suitable using smooth muscle cells
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推薦產品
生物源
mouse
品質等級
共軛
unconjugated
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
1A4, monoclonal
分子量
antigen ~42 kDa
包含
15 mM sodium azide
物種活性
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
技術
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells
immunohistochemistry (frozen sections): suitable using smooth muscle cells
indirect immunofluorescence: 1:400 using blood vessels in sections of human appendix
western blot: suitable using smooth muscle cells
同型
IgG2a
UniProt登錄號
應用
research pathology
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)
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一般說明
其抗体（也称为抗α-Sm-1）对α-平滑肌肌动蛋白的单一异构体具有特异性。它在免疫印迹试验中可与α-平滑肌肌动蛋白发生特异性反应，并可在冷冻或福尔马林固定的、或石蜡包埋的组织切片中对平滑肌细胞进行标记。
激动蛋白是可调节细胞运动的细胞骨架蛋白。数种支架、信号传导和肌动蛋白结合蛋白都可调节丝状F-肌动蛋白和单体G-肌动蛋白之间的相互转换。不同物种的细胞肌动蛋白具有非常相似的免疫和物理特性。因此，肌动蛋白单克隆抗体的使用为研究肌动蛋白的定位、结构和功能提供了一种特异且有用的工具。肌动蛋白 2 (ACTA2)，平滑肌肌动蛋白由人类染色体 10q23.31 编码。
小鼠抗肌动蛋白,α-平滑肌单克隆抗体可与人、小鼠、大鼠、牛、鸡、青蛙、山羊、豚鼠、兔、狗、绵羊和蛇的肌动蛋白结合。
抗-α-平滑肌肌动蛋白单克隆（小鼠IgM同种型）来源于小鼠骨髓瘤细胞与免疫小鼠脾细胞融合产生的杂交瘤。α-平滑肌肌动蛋白的NH2末端合成十肽与匙孔血蓝蛋白（KLH）偶联后可用作免疫原。该同种型是利用Sigma ImmunoTypeTM试剂盒（Sigma Stock No. ISO-1）以及通过小鼠单克隆抗体同种型试剂（Sigma Stock No. ISO-2）的双扩散免疫检测而进行确定的。
小鼠抗肌动蛋白,α-平滑肌单克隆抗体可与人、小鼠、大鼠、牛、鸡、青蛙、山羊、豚鼠、兔、狗、绵羊和蛇的肌动蛋白结合。
抗-α-平滑肌肌动蛋白单克隆（小鼠IgM同种型）来源于小鼠骨髓瘤细胞与免疫小鼠脾细胞融合产生的杂交瘤。α-平滑肌肌动蛋白的NH2末端合成十肽与匙孔血蓝蛋白（KLH）偶联后可用作免疫原。该同种型是利用Sigma ImmunoTypeTM试剂盒（Sigma Stock No. ISO-1）以及通过小鼠单克隆抗体同种型试剂（Sigma Stock No. ISO-2）的双扩散免疫检测而进行确定的。
特異性
单克隆抗-α 平滑肌Actin抗体具有针对单异构体α-actin的特异性。它可在福尔马林固定石蜡包埋或冷冻组织切片的免疫印迹及间接免疫荧光标记实验中特异性地与平滑肌肌动蛋白发生反应。该抗体可通过免疫过氧化物酶、免疫荧光、ELIS或免疫印迹技术用于α平滑肌肌动蛋白的免疫细胞化学定位。该抗体可与人、小鼠、大鼠、牛、鸡、青蛙、山羊、豚鼠、兔、狗、绵羊和蛇发生反应。
免疫原
N-末端合成的α-平滑肌肌动蛋白十肽。
應用
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
使用单克隆抗ACTA2抗体对来自牛主动脉的平滑肌细胞进行免疫细胞化学分析。首先将细胞在玻璃盖玻片上生长，并在4％的50％丙酮/乙醇中固定10分钟。
小鼠抗-α-平滑肌肌动蛋白单克隆抗体用于在人甲状腺未分化癌细胞中使用全肾匀浆液进行的免疫印迹检测。该产品还可用于对小鼠肝脏切片和子宫切片中的组织切片进行免疫组织化学测定。
抗α-平滑肌肌动蛋白单克隆抗体可用于通过免疫荧光染色法检测乳腺成纤维细胞中的肌动蛋白 2。
抗α-平滑肌肌动蛋白单克隆抗体可用于通过免疫荧光染色法检测乳腺成纤维细胞中的肌动蛋白 2。
生化／生理作用
肌动蛋白是最保守的真核蛋白之一，它可在哺乳动物和禽类中表达为通过电泳和氨基酸序列分析而得以表征的六种异构体。六种异构体中的四种可代表肌肉组织的分化标记物。肌动蛋白异构体显示>90%的整体序列同源性，但在其18个NH2-末端残基中只有50-60%的同源性。肌动蛋白的NH2-末端区域似乎是一个主要的抗原区域，可能参与了肌动蛋白与如肌球蛋白等其他蛋白的相互作用。已有证据表明，肌动蛋白异构体的相对比例在不同器官的平滑肌中是不同的，并且在发育、病理情况和不同培养条件下在相同的平滑肌细胞群体内也将发生变化。
肌动蛋白 2 的突变与动脉平滑肌功能障碍及冠心病相关。ACTA2中的突变会促进胸主动脉瘤（TAAD）中主动脉的撕裂和扩大。
肌动蛋白 2 的突变与动脉平滑肌功能障碍及冠心病相关。ACTA2中的突变会促进胸主动脉瘤（TAAD）中主动脉的撕裂和扩大。
外觀
该抗体以含有15mM叠氮化钠作为防腐剂的腹水形式提供。
儲存和穩定性
如需持续使用，可储存在 2-8 °C最长达一个月。如需储存更长时间，溶液可按工作体积分装后冻存。不建议反复冻融。如果在更长时间的储存后出现了轻微的浑浊，在使用前可通过离心而使其澄清。
其他說明
如需查看Actin抗体选择指南，请访问www.sigmaaldrich.com/actin。
免責聲明
除非在我们的产品目录或产品随附的其他公司文档中另有说明，我们的产品仅供研究使用并不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途、任何类型的食用或应用于人或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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分析證明 (COA)
Lot/Batch Number
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Does this antibody work for IF staining of cultured cells on a cover slip? What secondary would you recommend?
1 answer-
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Which is the suggested concentration for IHC in FF tissue?
1 answer-
The recommended concentration will be listed on the lot-specific Certificate of Analysis. For example, lot 0000221557 has an optimum concentration of 1:800.
Please see the sample Certificate of Analysis for more information: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A2547-.2ML0000246371.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of actin, Products A2066 and A2547?
1 answer-
Actin has a molecular weight of 42 kDa.
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Can Product A2547, Monoclonal Anti-Actin, α-Smooth Muscle antibody detect beta actin?
1 answer-
No, Product A2547, Monoclonal Anti-Actin, α-Smooth Muscle antibody will not cross react with beta actin.
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Is there a protocol for using Product A2547, Monoclonal Anti-Actin, α-Smooth Muscle antibody produced in mouse, in immunohistochemistry?
1 answer-
This antibody is used in our Immunohistochemistry Kit, Product No. IMMH2.
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What is the antibody concentration of Product A2547, Monoclonal Anti-Actin, α-Smooth Muscle antibody produced in mouse?
1 answer-
This antibody is ascities fluid. The amount of protein present and the amount of IgG1 present is indicated on the Lot-specific Certificate of Analysis.
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Why do I have 3 bands on my western blot when using Product A2547, Monoclonal Anti-Actin, α-Smooth Muscle antibody produced in mouse?
1 answer-
While actin should run as a 42 kDa band when detected by Western blotting, there are recent observations that cleavage products can be seen in cells undergoing apoptosis. Initial cleavage gives a band at 41 kDa, which may appear as a doublet on the blot. In addition, further cleavage results in a major band at 30 kDa and a small band at 14 kDa which have been shown to be cleavage products due to the action of interleukin 1 -converting enzyme. This may explain the detection of smaller bands when blotting with this antibody. This banding pattern may be detected with other actin antibodies as well. Kayalar, C., et al., Proc. Natl. Acad. Sci., USA, 93:2234-2238, (1996).
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