跳轉至內容
Merck
全部照片(1)

重要文件

43815

Sigma-Aldrich

DL-二硫苏糖醇

BioUltra, for molecular biology, ≥99.5% (RT)

同義詞:

(±)-二硫苏糖醇,rac-二硫苏糖醇,二硫苏糖醇, 1,4-二巯基苏糖醇, DTT, 克莱兰氏试剂

登入查看組織和合約定價


About This Item

線性公式:
HSCH2CH(OH)CH(OH)CH2SH
CAS號碼:
分子量::
154.25
Beilstein:
1719757
EC號碼:
MDL號碼:
分類程式碼代碼:
12161504
PubChem物質ID:
NACRES:
NA.25
暫時無法取得訂價和供貨情況

等級

for molecular biology

品質等級

產品線

BioUltra

化驗

≥99.5% (RT)

形狀

powder

反應適用性

reagent type: reductant

雜質

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.3% 4,5-dihydroxy-1,2-dithiane

pH值

4.0-6.5 (25 °C, 0.1 M in H2O)

mp

41-44 °C (lit.)

溶解度

H2O: 0.1 M at 20 °C, clear, colorless

負離子痕跡

sulfate (SO42-): ≤50 mg/kg

正離子痕跡

Al: ≤5 mg/kg
As: ≤0.5 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤500 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

&lambda ;

0.1 M in H2O

紫外吸收

λ: 260 nm Amax: 0.400
λ: 280 nm Amax: 0.100

SMILES 字串

O[C@H](CS)[C@H](O)CS

儲存溫度

2-8°C

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI 密鑰

VHJLVAABSRFDPM-QWWZWVQMSA-N

尋找類似的產品? 前往 產品比較指南

一般說明

二硫苏糖醇(DTT)是一种巯基还原剂,可定量还原生物分子中的二硫键(SH)。可特异性作用于交联剂N,N′-双(丙烯酰基)胱胺的二硫键桥,分解聚丙烯酰胺凝胶基质。DTT的刺激性和毒性都低于2-巯基乙醇。通常,使用DTT所需的浓度(100mM)是使用2-巯基乙醇(5% v/v, 700mM)浓度的七分之一,并且效力更强。

應用

二硫苏糖醇用途:
  • 作为酶稳定剂,维持胞壁水解酶exebacase的稳定性[1]
  • 用于蛋白提取和蛋白变性[2]
  • 用于质谱前的样品制备和甲醛交联逆转[3]
  • 作为缓冲液组分,提取synaptogliosome[4]
用于将 −SH 基保持在还原态的绝佳试剂;定量还原二硫化物。在 SDS-PAGE 前,DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常,使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。

生化/生理作用

二硫苏糖醇(DTT)广泛用于多肽化学合成和巯基蛋白的生化制备。另外,在蛋白折叠和蛋白酶活研究中也用到DTT。二硫苏糖醇可完全还原生物分子内或分子间的二硫键,直接介导巯基-二硫键交换反应。反应使生物分子生成巯基,DTT形成环状二硫键。[5]

特點和優勢

  • BioUltra级粉末,适用于分子生物学研究
  • 不含DNA酶、RNA酶和蛋白酶 不溶物通过过滤检测
  • 经过严格检测,不含微量金属

其他說明

如需了解生化试剂系列的更多信息,请填写此表
使用胍盐进行RNA的分离。包含还原剂通过破坏分子内的蛋白质二硫键来增强变性。[6]

象形圖

CorrosionExclamation mark

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

未看到正確版本?

如果您需要一個特定的版本,您可以透過批號來尋找特定憑證。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Immunoprecipitation of Ribosome-Bound mRNAs from Astrocytic Perisynaptic Processes of the Mouse Hippocampus
Mazare N, et al.
STAR protocols (2022)
Generation of cell-type-specific proteomes of neurodevelopment from human cerebral organoids
Melliou S and Diamandis P
STAR protocols (2022)
Jun T Oh et al.
Antimicrobial agents and chemotherapy, 65(7), e0258720-e0258720 (2021-04-28)
Exebacase (CF-301) belongs to a new class of protein-based antibacterial agents, known as lysins (peptidoglycan hydrolases). Exebacase, a novel lysin with antistaphylococcal activity, is in phase 3 of clinical development. To advance into the clinic, it was necessary to develop
John C Lukesh et al.
Journal of the American Chemical Society, 134(9), 4057-4059 (2012-02-23)
Dithiothreitol (DTT) is the standard reagent for reducing disulfide bonds between and within biological molecules. At neutral pH, however, >99% of DTT thiol groups are protonated and thus unreactive. Herein, we report on (2S)-2-amino-1,4-dimercaptobutane (dithiobutylamine or DTBA), a dithiol that
Isolation of RNA using guanidinium salts.
R J MacDonald et al.
Methods in enzymology, 152, 219-227 (1987-01-01)

文章

Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.

瞭解 mRNA 疫苗如何誘發免疫力。閱讀合成 mRNA 如何製備疫苗免疫原和其他生物製藥。尋找合成 mRNA 的試劑。

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is it normal for an IgG1 solution to become milky after DTT reduction?

    1 answer
    1. It is uncommon for a reducing agent to cause protein precipitation. However, in some cases, the reduction of a protein with a reducing agent like DTT can result in a change in the isoelectric point of the protein or protein fragments. This change in pH may create conditions where precipitation of the protein is more likely to occur.

      Helpful?

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務