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B5655

Sigma-Aldrich

BCIP®/NBT

SIGMAFAST™ BCIP®/NBT

SIGMAFAST™, alkaline phosphatase substrate, chromogenic, tablet

同義詞:

BCIP®/NBT碱性磷酸酶底物

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83
暫時無法取得訂價和供貨情況

產品名稱

SIGMAFAST™ BCIP®/NBT, tablet

形狀

tablet

溶解度

water: 10 mL, clear, yellow

儲存溫度

−20°C

相關類別

應用

SIGMAFAST BCIP®/NBT已被用作以下底物：
  • 用于免疫印迹的抗小鼠IgG偶联碱性磷酸酶[1]
  • 用于原代成骨细胞组织化学染色中碱性磷酸酶[2]
  • 骨髓基质细胞碱性磷酸酶染色[3]

重構

将一片溶于10 ml去离子水中，得到含有BCIP/NBT的即用型缓冲溶液，pH 9.5。

法律資訊

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 2

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000411613
0000411612
0000411612
0000411613
0000411215

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Mitogen-activated protein kinase phosphatase 1 regulates bone mass, osteoblast gene expression, and responsiveness to parathyroid hormone
Mahalingam CD, et al.
The Journal of Endocrinology, 211(2), 145-156 (2011)
MicroRNA-183-5p increases with age in bone-derived extracellular vesicles, suppresses bone marrow stromal (stem) cell proliferation, and induces stem cell senescence
Davis C, et al.
Tissue Engineering: Part A, 23(21-22), 1231-1240 (2017)
R Gonzalez-Castro et al.
Inflammopharmacology, 26(3), 817-827 (2017-11-03)
The main amyloid-beta (Aβ) variants detected in the human brain are full-length Aβ1-40 and Aβ1-42 peptides; however, a significant proportion of AD brain Aβ consists also of N-terminal truncated/modified species. The majority of the previous immunotherapeutic strategies targeted the N-terminal
Nicolas Leventoux et al.
Stem cell research, 47, 101896-101896 (2020-07-14)
Amyotrophic Lateral Sclerosis is the most common motor neuron degenerative disease in adults, and TARDBP gene mutations have been reported to be involved in the pathogenesis. We present here how we generated the human induced pluripotent stem cell (hiPSC) line
R Garg et al.
Human vaccines & immunotherapeutics, 13(12), 2894-2901 (2017-08-22)
Respiratory syncytial virus (RSV) is the most common cause of respiratory tract infections in both children and elderly people. In this study we evaluated the short- and long-term protective efficacy of a single intranasal (IN) immunization with a RSV vaccine
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文章

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

條款

Immunodetection Using BCIP/NBT Substrate

BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

使用 BCIP/NBT 底物進行免疫檢測

BCIP/NBT 底物可為鹼性磷酸酶檢測產生穩定、濃豔的顏色，非常適合目視觀察。

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

免疫組織化學程序

使用本方案，可在石蠟包埋組織切片中透過染色和顯現特定抗原，進行整個免疫組織化學 (IHC) 程序。

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Questions

  1. Is it ok to store this tablet (sigmafast BCIP/NBT) after being dissolved in water? (in case I couldn't use all 10 mL) If it's ok, which temperature (and other conditions) should I store it?

    1 answer

    1. For best results, it is recommended to use the solution within 1 hour. Long term storage of the solution has not been evaluated. A prepared BCIP/NBT solution is available under product number B6404. This product is prepared in Tris buffer and may be stored at room temperature. See the links below for the product datasheets for each item.

      B5655 Tablets
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/403/473/b5655dat.pdf

      B6404 Premixed Solution
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/116/719/b6404bul.pdf

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