推薦產品
生物源
goat
重組細胞
expressed in goat
共軛
alkaline phosphatase conjugate
抗體表格
affinity isolated antibody
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
物種活性
mouse
應無反應活性
human
技術
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25
western blot: 1:50,000-1:100,000 using total cell extract of HeLa cells
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
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一般說明
IgG 抗体在补体激活、吞噬作用、胎盘转运和细胞表面受体结合等体液免疫应答中起关键作用。抗小鼠 IgG（Fc 特异性）-碱性磷酸酶抗体（以1：10,000 的稀释度采用PBS-Tween 稀释）可用于 ELISA。它也可用于免疫印迹。山羊抗小鼠 IgG（Fc 特异性）-碱性磷酸酶抗体与小鼠 IgG Fc 片段、IgG 及其亚类 IgG1、IgG2a、IgG2b 和 IgG3 发生特异性反应，但不与人 IgG 和小鼠 IgA 或 IgM 发生反应。
免疫原
纯化的小鼠 IgG Fc 片段。
應用
抗小鼠 IgG（Fc 特异性）-碱性磷酸酶抗体可用于 ELISPOT（免疫斑点）测定。它也可用于免疫组化和蛋白质印迹。
碱性磷酸酶缀合的山羊抗小鼠 Fc 特异性抗体在 ELISA 测定中用作二抗，在 PBS/0.1% Tween 和 1% BSA中以 1:1000 的稀释度，在 37°C 下持续 1.5 小时。该抗体经开发使用磷酸 4-硝基苯酯（Sigma）作为底物，在 37°C 下，持续 30分钟。
其他說明
用人IgG吸附抗体。
外觀
0.05 MTris (pH 8.0) 溶液，含 1% 牛血清白蛋白、1 mM MgCl 2 和 15 mM 叠氮化钠
準備報告
经过吸附以减少人体样本的背景染色。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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分析證明 (COA)
Lot/Batch Number
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文章
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
ELISpot 分析可提供免疫反應的定性與定量資訊，可顯示單一反應細胞的多種分泌產物。
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