The efficiency of CelLytic Y reagent for protein extraction has been tested on different strains of Saccharomyces cerevisiae [Y187, Y190, W303(a), S288C, SP1, S1278b (diploid and haploid), BJ2168, and cdc25] and Schizosaccharomyces pombe.
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品質等級
形狀
solution
一般說明
特點和優勢
- 快速:在 20 min 内实现细胞裂解
- 效率:比玻璃珠高三倍
- 柔性:加入还原剂 (DTT) 后裂解增强
- 温和:非变性-保留生物活性
法律資訊
訊號詞
Warning
危險聲明
危險分類
Aquatic Chronic 3 - Eye Irrit. 2
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
從最近期的版本中選擇一個:
分析證明 (COA)
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文章
CelLytic Y 提供不含磷酸鹽的酵母細胞裂解,而 CelLytic Y 酵素套件則可確保從各種酵母菌株中有效萃取蛋白質。
CelLytic Y offers phosphate-free yeast cell lysis, while CelLytic Y Enzymatic Kit ensures efficient protein extraction from various yeast strains.
ReadyShield® 磷酸酶和蛋白酶抑制劑雞尾酒常見問題,用於各種細胞類型和組織萃取物的樣本保護,包括哺乳動物、植物和微生物樣本。我們的 ReadyShield® Protease Inhibitor Cocktail 是一種非冷凍溶液,含有對絲氨酸、半胱氨酸、酸性蛋白酶和氨肽酶具有廣泛特異性的抑制劑。
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
相關內容
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
細胞裂解與蛋白質萃取方法概述各種技術,從洗滌劑溶解到機械破壞,支援研究需求。
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What strains have been used with CelLytic™ Y Cell Lysis Reagent, Product C4482?
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Should I use CelLytic™ Y Cell Lysis Reagent, Product C4482?
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For all cases in which the addition of DTT does not interfere with the application tested, it is recommended to add DTT (5 - 10 mM final concentration) to the CelLytic Y reagent. Addition of DTT significantly improves total protein yield.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can I use CelLytic™ Y Cell Lysis Reagent, Product C4482, to make spheroplasts?
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No. For spheroplast formation and protein extraction we recommend the CelLytic Y Plus kit.
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What is in CelLytic™ Y Cell Lysis Reagent, Product C4482?
1 answer-
The formulation is considered proprietary, but we can state that it is a non-denaturing phosphate-free reagent.
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Can I use glass beads with CelLytic™ Y Cell Lysis Reagent, Product C4482?
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If cell growth conditions are not optimal, then glass beads can be used to increase yields.
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