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C4482

Sigma-Aldrich

CelLytic Y 细胞裂解试剂

For yeast cells

同義詞:

Yeast cell lysis reagent

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About This Item

分類程式碼代碼:
41116134
NACRES:
NA.56
暫時無法取得訂價和供貨情況

品質等級

形狀

solution

一般說明

CelLytic Y 细胞裂解试剂具有通用性、无磷酸盐和非变性。为细胞裂解和蛋白溶解提供了有效的方法。维持免疫反应性或生物学功能的靶蛋白。方案简短,在室温下执行。无需极端条件或玻璃珠。仅使用 2.5-5 mL/g 酵母细胞。

應用

CelLytic Y 细胞裂解试剂已用于裂解表达 DNA 修复、Rad 蛋白、[1] 热休克蛋白 (ssa1) [2] 和人白细胞介素 4 (hIL4) 的酵母细胞。[3]

特點和優勢

  • 快速:在 20 min 内实现细胞裂解
  • 效率:比玻璃珠高三倍
  • 柔性:加入还原剂 (DTT) 后裂解增强
  • 温和:非变性-保留生物活性

法律資訊

CelLytic is a trademark of Sigma-Aldrich Co. LLC

象形圖

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訊號詞

Warning

危險聲明

危險分類

Aquatic Chronic 3 - Eye Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


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存取文件庫

The beta6beta7 region of the Hsp70 substrate-binding domain mediates heat-shock response and prion propagation
Xu L, et al.
Cellular and Molecular Life Sciences, 75(8), 1445-1459 (2018)
Expression of recombinant human IL-4 in Pichia pastoris and relationship between its glycosylation and biological activity
Li R, et al.
Protein Expression and Purification, 96, 1-7 (2014)
DNA damage induced nucleotide excision repair in Saccharomyces cerevisiae
Singh RK and Krishna M
Molecular and Cellular Biochemistry, 290(1-2), 103-112 (2006)

文章

CelLytic Y 提供不含磷酸鹽的酵母細胞裂解,而 CelLytic Y 酵素套件則可確保從各種酵母菌株中有效萃取蛋白質。

CelLytic Y offers phosphate-free yeast cell lysis, while CelLytic Y Enzymatic Kit ensures efficient protein extraction from various yeast strains.

ReadyShield® 磷酸酶和蛋白酶抑制劑雞尾酒常見問題,用於各種細胞類型和組織萃取物的樣本保護,包括哺乳動物、植物和微生物樣本。我們的 ReadyShield® Protease Inhibitor Cocktail 是一種非冷凍溶液,含有對絲氨酸、半胱氨酸、酸性蛋白酶和氨肽酶具有廣泛特異性的抑制劑。

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

相關內容

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

細胞裂解與蛋白質萃取方法概述各種技術,從洗滌劑溶解到機械破壞,支援研究需求。

Questions

1–6 of 6 Questions  
  1. What strains have been used with CelLytic™ Y Cell Lysis Reagent, Product C4482?

    1 answer
    1. The efficiency of CelLytic Y reagent for protein extraction has been tested on different strains of Saccharomyces cerevisiae [Y187, Y190, W303(a), S288C, SP1, S1278b (diploid and haploid), BJ2168, and cdc25] and Schizosaccharomyces pombe.

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  2. Should I use CelLytic™ Y Cell Lysis Reagent, Product C4482?

    1 answer
    1. For all cases in which the addition of DTT does not interfere with the application tested, it is recommended to add DTT (5 - 10 mM final concentration) to the CelLytic Y reagent.  Addition of DTT significantly improves total protein yield.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Can I use CelLytic™ Y Cell Lysis Reagent, Product C4482, to make spheroplasts?

    1 answer
    1. No.  For spheroplast formation and protein extraction we recommend the CelLytic Y Plus kit.

      Helpful?

  5. What is in CelLytic™ Y Cell Lysis Reagent, Product C4482?

    1 answer
    1. The formulation is considered proprietary, but we can state that it is a non-denaturing phosphate-free reagent.

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  6. Can I use glass beads with CelLytic™ Y Cell Lysis Reagent, Product C4482?

    1 answer
    1. If cell growth conditions are not optimal, then glass beads can be used to increase yields.

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