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推薦產品
生物源
bacterial (Arthrobacter luteus)
品質等級
形狀
lyophilized powder
比活性
≥200 units/mg solid
技術
cell based assay: suitable
適合性
suitable for cell lysis
應用
diagnostic assay manufacturing
儲存溫度
−20°C
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應用
藤黄节杆菌(Arthrobacter luteus)溶壁酶可用于重悬细胞以提取高分子量DNA用于PCR（聚合酶链式反应）指纹分析。
生化／生理作用
溶壁酶(Lyticase)常用于真菌研究，特别是用于PCR法（聚合酶链式反应）物种鉴定。可切开糖基之间的β (1→3)和β (1→4)键。
溶壁酶是一种裂解酶，用于通过诱导部分原生质球形成，从酵母细胞中提取DNA。[3]随后再裂解原生质球以释放DNA。酵母细胞的细胞壁可能形成荚膜或耐药孢子，很难破坏，因此溶壁酶成为消化酵母细胞壁的首选方案。溶壁酶含有β-(1→;3)-葡聚糖昆布五糖水解酶以及β-(1→3)-葡聚糖酶、蛋白酶和甘露聚糖酶活性。[4]它适用于多种酵母细胞，例如念珠菌(Candida)、德巴利酵母菌([5] Debaryomyces)、酿酒酵母(Saccharomyces)、覆膜酵母(Saccharomycopsis)、类酵母酵(Saccharomycodes)、出鞘菌属(Eremothecium)和许旺酵母(Schwanniomyces species)。
溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。
單位定義
一个单位在25°C和pH 7.5条件下，在3ml反应混合物中以酵母悬浮液为底物，每分钟产生0.001的ΔA800 。
其他說明
仅供研发使用。&不可用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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條款
本程序可用於以 Baker's yeast 為底物的溶酶活性測定。
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
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