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Merck
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重要文件

324506

Millipore

EDTA,0.5 M,pH 8.0,分子生物学级,DEPC 处理

Sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). Suitable for use in molecular biology applications.

同義詞:

EDTA,0.5 M,pH 8.0,分子生物学级,DEPC 处理, 乙二胺四乙酸三钠盐

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About This Item

經驗公式(希爾表示法):
C10H13N2Na3O8 · 3H2O
CAS號碼:
分子量::
412.23
MDL號碼:
分類程式碼代碼:
12352107
NACRES:
NA.25
暫時無法取得訂價和供貨情況

等級

Molecular Biology

品質等級

形狀

liquid

反應適用性

reagent type: chelator

製造商/商標名

Calbiochem®

儲存條件

OK to freeze

pH值

8

運輸包裝

ambient

儲存溫度

15-25°C

SMILES 字串

[Na+].[Na+].[Na+].N(CCN(CC(=O)[O-])CC(=O)O)(CC(=O)[O-])CC(=O)[O-].O.O.O

InChI

1S/C10H16N2O8.3Na.3H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;3*1H2/q;3*+1;;;/p-3

InChI 密鑰

FXNQQEVEDZAAJM-UHFFFAOYSA-K

一般說明

用碳酸氢二乙酯(DEPC)处理的无菌过滤的 0.5 M EDTA H2O 溶液。适用于分子生物学应用。

應用

  • 比较提取方法对高通量古蛋白组骨骼物种鉴定的效率。:该研究比较了多种用于古蛋白质组分析的提取方法,包括使用EDTA的方法,以有效地鉴定骨骼种类。该研究强调了EDTA在蛋白质组学研究中增强蛋白质提取的重要性(Mylopotamitaki et al., 2023)。

  • 骨组织蛋白质组的深度覆盖和定量为骨骼生物学和疾病的新发现提供了更多的机会。:讨论了EDTA在制备和提取用于蛋白质组学分析的骨样品中的应用,强调了其在实现蛋白质组学深度覆盖、促进骨骼生物学新发现中的作用(Rose et al., 2023)。

警告

毒性:标准处理(A)

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Health hazardCorrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - STOT RE 2 Inhalation

標靶器官

Respiratory Tract

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


分析證明 (COA)

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Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Hi, Is the DEPC innactivated in this solution ? I have some leftover and I am wondering if it would be suitable for use in a cell culture buffer context ? Thank you in advance for your support, Kind regards, Coline

    1 answer
    1. Yes, DEPC was inactivated. Note that one of the break down products of DEPC is ethanol. DEPC-treated solutions are typically not used in cell culture applications and may be cytotoxic.

      Helpful?

  4. Dear all, Is the EDTA 0.5 M solution prepared with before DEPC treated H2O, or, is it prepared together with DEPC and then autoclaved? Many thanks for your answer! Best, Julia

    1 answer
    1. The solution is prepared with DEPC-treated H2O.

      Helpful?

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