跳轉至內容
Merck
全部照片(2)

文件

E7889

Sigma-Aldrich

乙二胺四乙酸 二钠盐 溶液

for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

同義詞:

EDTA 二钠盐, EDTA-Na2, 亚乙基二次氮基四乙酸 二钠盐

登入查看組織和合約定價
全部照片(2)

About This Item

線性公式:
[-CH2N(CH2CO2Na)CH2CO2H]2
CAS號碼:
139-33-3
分子量：:
336.21
Beilstein:
3822669
MDL號碼:
MFCD00070672
分類程式碼代碼:
12352107
PubChem物質ID:
24894660
NACRES:
NA.31

等級

for molecular biology

品質等級

300

無菌

0.2 μm filtered

形狀

liquid

反應適用性

reagent type: chelator

濃度

0.5 M in H2O

pH值

7.5-8.5

適合性

suitable for molecular biology

應用

microbiology

異物活動

DNase, RNase, NICKase and protease, none detected

SMILES 字串

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

InChI 密鑰

ZGTMUACCHSMWAC-UHFFFAOYSA-L

尋找類似的產品？ 前往 產品比較指南

相關類別

一般說明

乙二胺四乙酸二钠盐溶液是一种螯合剂，适用于分子生物学应用。
乙二胺四乙酸（EDTA）是一种多元酸。EDTA中带孤对电子的四个羧酸和两个胺基可以螯合钙离子及其他几种金属离子。

應用

乙二胺四乙酸二钠盐溶液已应用于：
  • 盐酸（HCL）中消化蛋白酶-K，以测定角膜样本中DNA的含量
  • 与木瓜蛋白酶结合消化角膜样本，以量化糖胺聚糖（GAG）的含量
  • 预处理中，以剥离生长至80%融合的上皮细胞
  • 制备裂解缓冲液的原液
  • 制备尿素裂解缓冲液

特點和優勢

分子生物学级

準備報告

用分子生物学试剂 EDTA (E 5134) 在 18 兆欧水中制备

其他說明

如需了解生化试剂系列的更多信息，请填写此表。

象形圖

Health hazard
GHS08

訊號詞

Warning

危險聲明

H373

防範說明

P260 - P314 - P501

危險分類

STOT RE 2 Inhalation

標靶器官

Respiratory Tract

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

Choose from one of the most recent versions:

分析證明 (COA)

Lot/Batch Number
0000384159
0000387324
0000392905
0000390872
0000364461

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Marta Filipiak et al.
Fish physiology and biochemistry, 38(2), 355-362 (2011-05-27)
The aim of this study was to compare DNA content in hepatocyte and erythrocyte nuclei of the European sunbleak, Leucaspius delineatus, in relation to nuclear and cell size by means of flow cytometry and fluorescence microscopy. The DNA standards, chicken
Yi-Yuan Ke et al.
Foods (Basel, Switzerland), 10(1) (2021-01-21)
Isothiocyanates (ITCs) are important functional components of cruciferous vegetables. The principal isothiocyanate molecule in broccoli is sulforaphane (SFN), followed by erucin (ERN). They are sensitive to changes in temperature, especially high temperature environments where they are prone to degradation. The
Gary Reynolds et al.
Science (New York, N.Y.), 371(6527) (2021-01-23)
The skin confers biophysical and immunological protection through a complex cellular network established early in embryonic development. We profiled the transcriptomes of more than 500,000 single cells from developing human fetal skin, healthy adult skin, and adult skin with atopic
Yongchao Dou et al.
Cell, 180(4), 729-748 (2020-02-16)
We undertook a comprehensive proteogenomic characterization of 95 prospectively collected endometrial carcinomas, comprising 83 endometrioid and 12 serous tumors. This analysis revealed possible new consequences of perturbations to the p53 and Wnt/β-catenin pathways, identified a potential role for circRNAs in
Ci Chu et al.
Molecular cell, 44(4), 667-678 (2011-10-04)
Long noncoding RNAs (lncRNAs) are key regulators of chromatin state, yet the nature and sites of RNA-chromatin interaction are mostly unknown. Here we introduce Chromatin Isolation by RNA Purification (ChIRP), where tiling oligonucleotides retrieve specific lncRNAs with bound protein and
查看所有相關論文

文章

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

ReadyShield 磷酸酶和蛋白酶抑制劑混合液常見問題集

ReadyShield® 磷酸酶和蛋白酶抑制劑雞尾酒常見問題，用於各種細胞類型和組織萃取物的樣本保護，包括哺乳動物、植物和微生物樣本。我們的 ReadyShield® Protease Inhibitor Cocktail 是一種非冷凍溶液，含有對絲氨酸、半胱氨酸、酸性蛋白酶和氨肽酶具有廣泛特異性的抑制劑。

條款

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

相關內容

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務