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Merck
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重要文件

G7663

Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

同義詞:

blocking buffer

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About This Item

分類程式碼代碼:
41105300
NACRES:
NA.25
暫時無法取得訂價和供貨情況

等級

Molecular Biology
for molecular biology

形狀

powder blend

儲存溫度

room temp

一般說明

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

應用

Suitable for use as a non-specific blocking agent for Western blots.

重構

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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條款

為了特異性檢測固定在固體支持物上的抗原或目標分子,必須阻斷支持物上未被佔用的結合位點,防止探針和檢測分子結合。

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

學習 Northern 和 Southern 印迹的基本知識,以及將大分子轉移到膜支持物上的協議和應用。

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