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G1890

Sigma-Aldrich

Porcine Gelatin

from porcine skin, Type A, powder, gel strength ~300 g Bloom, suitable for electrophoresis and cell culture

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About This Item

CAS號碼:
9000-70-8
EC號碼:
232-554-6
MDL號碼:
MFCD00081638
分類程式碼代碼:
12352202
NACRES:
NA.75
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產品名稱

明胶 来源于猪皮肤, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture

生物源

Porcine skin

品質等級

200

無菌

sterile

種類

Type A

產品線

BioReagent

形狀

powder

分子量

50—100 kDa

包裝

pkg of 1 kg
pkg of 100 g
pkg of 500 g

濃度

70—90% Biuret

技術

cell culture | mammalian: suitable
electrophoresis: suitable

表面覆盖率

100‑200 μg/cm2

溶解度

H2O: soluble 50 mg/mL, clear to hazy, faintly yellow

運輸包裝

ambient

儲存溫度

room temp

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一般說明

来自猪皮的明胶是由胶原蛋白的酸消化产生，被称为 A 型。猪明胶主要包含甘氨酸、脯氨酸和羟脯氨酸。[1]三股螺旋的胶原水解后形成无规卷曲的明胶。[2]它的 N 端序列不同于 B 型牛明胶。[3]

應用

明胶已用于多种应用。它可用于对细胞培养器皿进行包被以改善细胞的附着，[4][5]加入至PCR以稳定Taq DNA，作为Western blotting、ELISA和免疫化学中的封闭试剂，以及作为细菌学中用于物种分化培养基的组分。作为一种生物相容性的聚合物，它已被作为递送载体用于活性生物分子的释放以及支架的生成用于组织工程应用。[6]在制药工业中，明胶可用作悬浮和包封剂，以及[7]其他应用。建议将1-5 μg/cm2或0.5-50 μg/ml的本品用作细胞培养基质。最佳的浓度的确取决于细胞类型以及应用和研究目标。

特點和優勢

  • 无菌明胶来源于猪皮。
  • 不含蛋白酶。
  • 粉末形式的明胶处理方便简单。
  • 可用于细胞培养和电泳技术。
  • 为各种应用提供足够的表面覆盖率。

成分

明胶存在于胶原蛋白中，是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。

注意

干燥的明胶，当在室温下储存于密闭容器中时，将可保持多年不变。 当在空气存在时在100℃下加热时，它会溶胀变软，并分解成碳质物质并伴有吡啶碱和氨的释放。

準備報告

该产品源自于猪皮。明胶可溶于热水而非冷水。 它几乎不溶于大多数有机溶剂，如醇、氯仿、二硫化碳、四氯化碳、乙醚、苯、丙酮和油。 由Bloom凝胶计所测定的Bloom指数，可表示由已知浓度溶液而形成的凝胶的强度。 Bloom指数与平均分子量成正比。 猪皮明胶的Bloom指数变化于90至300 g之间。该产品具有大约300的凝胶强度。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

nwg

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000410027
0000410026
0000410024
0000410026
0000410027

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存取文件庫

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Cheng YH, et al.
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The effects of GDF-5 and uniaxial strain on mesenchymal stem cells in 3-D culture
Farng E, et al.
Clinical Orthopaedics and Related Research, 466(8), 1930-1937 (2008)
Małgorzata Kozikowska et al.
Scientific reports, 9(1), 11969-11969 (2019-08-21)
The retention of the embryo in the transfer catheter after embryo transfer (ET) during in vitro fertilization is a very common phenomenon, encountered by even the most experienced operators, and embryos retained in the transfer catheter or its sleeve require
Graziano Martello et al.
The EMBO journal, 32(19), 2561-2574 (2013-08-15)
Self-renewal of pluripotent mouse embryonic stem (ES) cells is sustained by the cytokine leukaemia inhibitory factor (LIF) acting through the transcription factor Stat3. Several targets of Stat3 have previously been identified, most notably the reprogramming factor Klf4. However, such factors
In vitro derivation of chondrogenic cells from human embryonic stem cells
Human Embryonic Stem Cell Protocols, 77(1), 317-331 (2009)
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文章

Photo-Crosslinkable Gelatin Hydrogel: Versatile Materials for (High Resolution) Additive Manufacturing

Discussion of synthetic modifications to gelatin, improving the three-dimensional (3D) print resolution, and resulting material properties.

Attachment Factors for Three-dimensional Cell Culture

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

什麼是細胞外基質？

細胞外基質 (ECM) 由細胞分泌，包圍在組織中。

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條款

Gelatin Coating Protocol for Cell Culture

This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.

用於細胞培養的明膠塗層協議

這份用於細胞培養的明膠塗佈方案提供了明膠類型、表面覆蓋濃度以及最佳化技巧等相關資訊。

Questions

1–3 of 3 Questions  

  1. What is the molecular weight of this Gelatin?

    1 answer

    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

      Helpful?

  2. COULD YOU PERHAPS PROVIDE THE NUMBER OF CARBOXYLIC ACID GROUPS IN MOL PER 100 G OF PROTEIN

    1 answer

    1. According to published literature, Type A Gelatin has 78-80 millimoles of free carboxyl groups per 100 g of protein. Please see the link below to review the full product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/614/g6650-et-al-pis-ms.pdf

      Helpful?

  3. What is the molecular weight of gelatin from porcine skin, Type A?

    1 answer

    1. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin gelatin vary from 90 to 300 g. This product has a gel strength of approximately 300 g. Thus, the average molecular mass would be 50,000-100,000. Please review the Product Information Sheet for further information:

      https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/g1890pis.pdf

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