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重要文件

T9284

Sigma-Aldrich

Triton X-100

BioXtra

同義詞:

4-(1,1,3,3-四甲基丁基)苯基-聚乙二醇, t-辛基苯氧基聚乙氧基乙醇, 聚乙二醇 辛基苯醚

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About This Item

線性公式:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS號碼:
Beilstein:
2315025
MDL號碼:
分類程式碼代碼:
12161900
PubChem物質ID:
NACRES:
NA.21
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生物源

synthetic

品質等級

描述

non-ionic

產品線

BioXtra

形狀

liquid

分子量

micellar avg mol wt 80,000
average mol wt 625

凝集数

100-155

技術

permeability assay: suitable

雜質

≤0.05% Phosphorus (P)

燃燒殘留物

≤0.4%

CMC

0.2-0.9 mM (20-25°C)

轉變溫度

cloud point 65 °C
pour point ~7 °C

溶解度

water: soluble

密度

1.065 g/cm3 at 20 °C (68 °F)

負離子痕跡

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

正離子痕跡

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.1%
Pb: ≤0.001%
Zn: ≤0.0005%

HLB

13.5

SMILES 字串

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI 密鑰

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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一般說明

Triton X-100是一种常用的非离子型的表面活性剂和乳化剂,经常用于生化应用以溶解蛋白质。它被视为一种不变性的相对温和的洗涤剂,并且在许多参考文献中作为一种常规被添加的试剂而被报道。它用于裂解细胞以提取蛋白和细胞器。还可通透活细胞膜用于转染。[1][2]

應用

Triton X-100已用于:
  • 与Span® 80和Tween® 80一起用于矿物油中制备用于PCR技术的微乳液油相[3][4][5]
  • 用于在PCR循环后汇集和分解微乳液的NX缓冲液中的一种成分[3]
  • 在免疫细胞化学中用于封闭溶液中[6][7]
  • 在去糖基化过程中[8]
  • 膜的溶解[9]
  • 细胞的通透化处理[9][10]
  • 猪心脏瓣膜组织脱细胞过程[11]

生化/生理作用

广泛使用的非离子型表面活性剂,用于在温和的非变性条件下回收膜组分。

特點和優勢

  • BioXtra级别,适于细胞生物学和生物化学研究
  • 非离子型非变性去污剂
  • 优异的润湿剂和乳化剂
  • 多功能表面活性剂

法律資訊

Span is a registered trademark of Croda International PLC
TWEEN is a registered trademark of Croda International PLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

483.8 °F - closed cup

閃點(°C)

251 °C - closed cup

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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分析證明 (COA)

Lot/Batch Number

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存取文件庫

Angiotensin II AT1 receptor blockade decreases vasopressin-induced water reabsorption and AQP2 levels in NaCl-restricted rats.
Kwon TH et al.
American Journal of Physiology: Renal Physiology, 288, F673-F673 (2005)
Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations.
Dressman D, et al.
Proceedings of the National Academy of Sciences of the USA, 100(15), 8817-8822 (2003)
Proinflammatory chemokine induction in keratocytes and inflammatory cell infiltration into the cornea.
Hong JW et al.
Investigative Ophthalmology & Visual Science, 42, 2795-2795 (2001)
Structural, metabolic and developmental evaluation of ovulated rabbit oocytes before and after cryopreservation by vitrification and slow freezing.
Theriogenology, 74(5), 847-855 (2010)
Membrane and acto-myosin tension promote clustering of adhesion proteins.
Delanoe-Ayari H, et al.
Proceedings of the National Academy of Sciences of the USA, 101(8), 2229-2234 (2004)

條款

細胞染色可分為四個步驟:細胞製備、固定、施用抗體及評估。

Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

Questions

1–8 of 8 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Is this the Triton X-100 (T9284) considered sterile?

    1 answer
    1. This product is not sterile. Should this be a requirement, see product 648463, a 10% sterile solution of Triton X-100. https://www.sigmaaldrich.com//product/mm/648463

      Helpful?

  4. Hi, I wanna to ask how to make 0.1% triton. how much TritonX-100 I should use. Because I really can't understand what does X-100 mean.

    1 answer
    1. This item is considered 100 % Triton X-100. A 1000 x dilution can be used to make a 0.1% triton solution.

      Helpful?

  5. What's the surface tension of this product?

    1 answer
    1. T9284 is Triton X100 that comes from Dow Chemical Company. The surface tension is 33 dynes/cm at 1% actives, 25°C as per the technical data sheet on the vendor's website: https://www.dow.com/en-us/pdp.triton-x-100-surfactant.85735z.html#tech-content

      Helpful?

  6. What is the Surface Tension value of Triton X-100? and, what is the hydrophilic/lipophilic balance (HLB) and critical micelle concentration (cmc) of Triton X-100?

    1 answer
    1. • Cloud Point @1% Aqueous (ASTM D 2024): 66 °C
      • CMC (25°C): 189 PPM
      • Foam Height - Initial (0.1 wt% actives): 128 mm
      • Foam Height-5 minutes (0.1 wt% actives): 107 mm
      • HLB: 13.4 Moles EO: 9.5 Pour Point: 1 °C
      • Surface Tension (1% actives, 25 °C): 33 dynes/cm

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  7. What is the expiration date for the products?

    1 answer
    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 2.5 years after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  8. Hello, I bought the following product: Triton X-100 (T9284) - BioXtra and I was wondering what grade is it? Is it suitable for nucleic acid extraction? Thanks, Irene

    1 answer
    1. The BioXtra products are characterized by more stringent testing protocols that may include trace metal analysis and specialized suitability testing. This product is not tested specifically for nucleic acid extraction. However, there is no specific aspect of the product that would render it unsuited for this application. For future reference, the molecular biology grade (T8787) may be of interest. This product is tested for DNAse and RNAse activity. See the links below to review this product option:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T8787-BULKSLCJ6163.pdf
      https://www.sigmaaldrich.com/product/sigma/t8787

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