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重要文件

F8775

Sigma-Aldrich

甲醛 溶液

for molecular biology, 36.5-38% in H2O

同義詞:

福尔马林

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About This Item

線性公式:
HCHO
CAS號碼:
分子量::
30.03
Beilstein:
1209228
EC號碼:
MDL號碼:
分類程式碼代碼:
12352114
PubChem物質ID:
NACRES:
NA.31
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等級

Molecular Biology
for molecular biology

蒸汽密度

1.03 (vs air)

蒸汽壓力

52 mmHg ( 37 °C)

形狀

liquid

自燃溫度

572 °F

包含

10-15% methanol

濃度

36.5-38% in H2O

技術

ChIP: suitable
immunofluorescence: suitable
immunohistochemistry: suitable

密度

1.09 g/mL at 25 °C (lit.)

適合性

suitable for (use in Formaldehyde-Agarose Gel Electrophoresis of RNA)

應用

general analytical
genomic analysis

儲存溫度

15-25°C

SMILES 字串

[H]C([H])=O

InChI

1S/CH2O/c1-2/h1H2

InChI 密鑰

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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一般說明

甲醛是一种具有刺激性气味的无色化学物质,广泛应用于多种生物学研究和实验室环境。它主要以37%溶液(福尔马林)的形式提供,其多样化用途包括组织保存、杀菌和RNA凝胶电泳中的变性剂。但是,重要的是要认识到它的潜在健康风险,并以合适的预防措施进行处理。多样化功能:
  • 防腐剂:甲醛抑制微生物生长的能力令其成为生物学样品的有效防腐剂,防止腐败并确保分析用样品的完整性。这种特性的应用见于医学实验室、博物馆和防腐液。
  • 固定剂:在历史上,甲醛可用作固定剂,可交联蛋白并保护显微镜检查用组织的结构。这种特性让研究人员能够以良好的清晰度观察解剖学细节和细胞成分。
  • 变性剂:在分子生物学技术领域,如甲醛-琼脂糖凝胶电泳,甲醛使RNA分子变性,从而防止其形成二级结构,并实现基于尺寸的分离。这种技术对于基因表达和RNA完整性的研究有重要价值。

應用

甲醛溶液已用于固定细胞和组织切片。[1][2]它已用于甲醛琼脂糖凝胶电泳。[3]甲醛可用作RNA的甲醛-琼脂糖凝胶电泳中的变性剂。

特點和優勢

  • 用途广泛,适用于多种实验室和研究应用
  • 适用于分子生物学和生物化学应用

分析報告

针对用于 RNA 的甲醛-琼脂糖凝胶电泳进行了测试。

同類產品

產品號碼
描述
訂價

訊號詞

Danger

危險分類

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

標靶器官

Eyes,Central nervous system, Respiratory system

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

133.0 °F - closed cup

閃點(°C)

56.11 °C - closed cup


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存取文件庫

RNA-dependent RNA Polymerase Assay for Hepatitis E Virus.
Nair VP et al.
Bio-protocol null
Farrell RE
RNA Methodologies: A Laboratory Guide for Isolation and Characterization (2010)
Lun Zhao et al.
Nature communications, 10(1), 3640-3640 (2019-08-15)
Insight into high-resolution three-dimensional genome organization and its effect on transcription remains largely elusive in plants. Here, using a long-read ChIA-PET approach, we map H3K4me3- and RNA polymerase II (RNAPII)-associated promoter-promoter interactions and H3K9me2-marked heterochromatin interactions at nucleotide/gene resolution in rice.
Burry RW
Immunocytochemistry: A Practical Guide for Biomedical Research (2009)
Yanwei Sha et al.
Molecular genetics & genomic medicine, 7(8), e807-e807 (2019-07-04)
Asthenozoospermia (AZS), also known as asthenospermia, is characterized by reduced motility of ejaculated spermatozoa and is detected in more than 40% of infertile patients. Because the proportion of progressive spermatozoa in severe AZS is <1%, severe AZS is an urgent

條款

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

Sigma Imprint染色质免疫沉淀试剂盒利用检测板系统,可实现快速的高通量ChIP检测

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

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    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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