Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Advanced healthcare materials (2015-06-20)

Timothy M O'Shea, Matthew J Webber, Alex A Aimetti, Robert Langer

摘要

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong and -ordered hydrogen bonding interactions within covalently incorporated trehalose hydrogels represent a possible mechanism for protein stabilization.

材料

货号

品牌

产品描述

D2650

Sigma-Aldrich

二甲基亚砜, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

D8418

Sigma-Aldrich

二甲基亚砜, for molecular biology

D2438

Sigma-Aldrich

二甲基亚砜, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

D4540

Sigma-Aldrich

二甲基亚砜, ≥99.5% (GC), suitable for plant cell culture

276855

Sigma-Aldrich

二甲基亚砜, anhydrous, ≥99.9%

T9531

Sigma-Aldrich

D-(+)-海藻糖二水合物, ≥99% (HPLC), from Saccharomyces cerevisiae

270997

Sigma-Aldrich

二氯甲烷, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

H9892

Sigma-Aldrich

盐酸溶液, 1.0 N, BioReagent, suitable for cell culture

21115

Sigma-Aldrich

氯化钙溶液, BioUltra, for molecular biology, ~1 M in H₂O

H1758

Sigma-Aldrich

盐酸, 36.5-38.0%, BioReagent, for molecular biology

322415

Sigma-Aldrich

甲醇, anhydrous, 99.8%

T0167

Sigma-Aldrich

D-(+)-海藻糖二水合物, from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

C5670

Sigma-Aldrich

氯化钙, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

S7388

Sigma-Aldrich

N-琥珀酰-丙酰氨-丙酰氨-脯酰氨-苯丙氨酸对硝基酰苯胺

T5251

Sigma-Aldrich

D-(+)-海藻糖二水合物, ≥98.5% (HPLC), from Saccharomyces cerevisiae

T9449

Sigma-Aldrich

D-(+)-海藻糖二水合物, ≥99% (HPLC), from starch

41639

Sigma-Aldrich

二甲基亚砜, BioUltra, for molecular biology, ≥99.5% (GC)

860336

Sigma-Aldrich

3,3′,5,5′-四甲基联苯胺, ≥99%

2104

Supelco

盐酸溶液, volumetric, 0.1 M HCl (0.1N), endotoxin free

270989

Sigma-Aldrich

乙酸乙酯, anhydrous, 99.8%

L4777

Sigma-Aldrich

脂肪酶丙烯酸树脂, ≥5,000 U/g, recombinant, expressed in Aspergillus niger

499609

Sigma-Aldrich

氯化钙, anhydrous, powder, 99.99% trace metals basis

C3142

Sigma-Aldrich

α-糜蛋白酶来源于牛胰腺, (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

D9170

Sigma-Aldrich

二甲基亚砜, PCR Reagent

T2885

Sigma-Aldrich

3,3′,5,5′-四甲基联苯胺, ≥98% (TLC)

702129

Sigma-Aldrich

氧化铝, nanoparticles, <50 nm particle size (DLS), 20 wt. % in isopropanol

84429

Sigma-Aldrich

盐酸溶液, ~6 M in H₂O, for amino acid analysis

D1435

Sigma-Aldrich

二甲基亚砜, meets EP testing specifications, meets USP testing specifications

718475

Sigma-Aldrich

氧化铝, nanopowder, 13 nm primary particle size (TEM), 99.8% trace metals basis

705551

Sigma-Aldrich

氯化氢溶液, 3 M in cyclopentyl methyl ether (CPME)