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C5670

Sigma-Aldrich

氯化钙

anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

别名:

CaCl2, Calcium dichloride

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$213.00
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100 G
$213.00
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About This Item

线性分子式:
CaCl2
CAS号:
10043-52-4
分子量:
110.98
EC號碼:
233-140-8
MDL號碼:
MFCD00010903
分類程式碼代碼:
12352207
PubChem物質ID:
24892577
NACRES:
NA.71

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等級

anhydrous

品質等級

200

蒸汽壓力

0.01 mmHg ( 20 °C)

產品線

BioReagent

化驗

≥96.0%

形狀

powder

技術

cell culture | insect: suitable
cell culture | plant: suitable

mp

772 °C (lit.)

SMILES 字串

[Cl-].[Cl-].[Ca++]

InChI

1S/Ca.2ClH/h;2*1H/q+2;;/p-2

InChI 密鑰

UXVMQQNJUSDDNG-UHFFFAOYSA-L

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一般說明

氯化钙是一种常用的生化试剂。 钙在许多生物过程中起重要作用，如信号传导、肌肉收缩以及细胞膜和细胞壁的稳定性维持。1,2关于生物钙的实验测量已有大量文献发表。3,4氯化钙可用于感受态大肠杆菌的制备和转化，以及真核细胞的质粒DNA或高分子量基因组DNA转染。5已有研究显示质粒DNA pBR322可通过 CaCl2介导的电穿孔法导入大肠杆菌。6已有发表文献采用通过CaCl2浓缩病毒载体的实验方案。7CaCl2已用于大肠杆菌来源的NADH-泛醌氧化还原酶（复合体I）的稳定和二维结晶。8在CaCl2和柠檬酸钠存在下结晶的猪胰弹性蛋白酶显示其金属结合位点结合的是钙离子。9已通过多孔荧光法测定CaCl2对大肠杆菌微管蛋白样FtsZ分裂蛋白聚合化时总荧光强度的影响。10

應用

该产品已经过昆虫细胞培养测试(0.5 mg/L)，证明其适用于昆虫细胞培养。氯化钙可用于感受态大肠杆菌的制备和转化，
以及真核细胞的质粒DNA或高分子量基因组DNA转染。已有研究显示质粒DNA pBR322可通过CaCl2介导的电穿孔法导入大肠杆菌。已有发表文献采用通过CaCl2浓缩病毒载体的实验方案。

準備報告

该产品可溶于水（222 mg/ml），形成澄清无色溶液。

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H319

危險分類

Eye Irrit. 2

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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J Philippe et al.
International journal of obesity (2005), 39(2), 295-302 (2014-06-04)
A significant proportion of severe familial forms of obesity remain genetically elusive. Taking advantage of our unique cohort of multigenerational obese families, we aimed to assess the contribution of rare mutations in 29 common obesity-associated genes to familial obesity, and
Maheswari Jayakannan et al.
Journal of experimental botany, 66(7), 1865-1875 (2015-01-24)
The role of endogenous salicylic acid (SA) signalling cascades in plant responses to salt and oxidative stresses is unclear. Arabidopsis SA signalling mutants, namely npr1-5 (non-expresser of pathogenesis related gene1), which lacks NPR1-dependent SA signalling, and nudt7 (nudix hydrolase7), which
Mohsen Akbari et al.
Advanced functional materials, 24(26), 4060-4067 (2014-11-21)
The fabrication of cell-laden structures with anisotropic mechanical properties while having a precise control over the distribution of different cell types within the constructs is important for many tissue engineering applications. Automated textile technologies for making fabrics allow simultaneous control
Michael A Goren et al.
Nature communications, 5, 5115-5115 (2014-10-09)
Opsin, the rhodopsin apoprotein, was recently shown to be an ATP-independent flippase (or scramblase) that equilibrates phospholipids across photoreceptor disc membranes in mammalian retina, a process required for disc homoeostasis. Here we show that scrambling is a constitutive activity of
Naoya Nishitani et al.
The international journal of neuropsychopharmacology, 17(8), 1321-1326 (2014-05-24)
Several lines of evidence indicate that ketamine has a rapid antidepressant-like effect in rodents and humans, but underlying mechanisms are unclear. In the present study, we investigated the effect of ketamine on serotonin (5-HT) release in the rat prefrontal cortex
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商品

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

钙在细胞培养中的应用

钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。

Questions

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What is the procedure for preparing a 2 M solution using calcium chloride and calcium chloride dihydrate?

    1 answer

    1. The molecular weight for calcium chloride dihydrate is 147.01 g/mol, so a 2 M solution would be 294 g/L. Similarly, the molecular weight for calcium chloride is 110.98 g/mole, so a 2 M solution would be 222 g/L. Therefore, a 2 M solution of calcium chloride dihydrate (such as C7902) would be 294 g/L with a molecular weight of 147.01 g/mol.

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