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Merck

T0167

Sigma-Aldrich

D-(+)-海藻糖 二水合物

from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

别名:

α,α-海藻糖, α-D-吡喃葡萄糖基-α-D-吡喃葡萄糖苷

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10 G
$148.00
25 G
$241.00
100 G
$727.00

$148.00


预计发货时间2025年4月01日详情


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10 G
$148.00
25 G
$241.00
100 G
$727.00

About This Item

经验公式(希尔记法):
C12H22O11 · 2H2O
CAS号:
分子量:
378.33
Beilstein:
5322018
EC 号:
MDL编号:
UNSPSC代码:
12352201
PubChem化学物质编号:
NACRES:
NA.75

$148.00


预计发货时间2025年4月01日详情


获取大包装报价

质量水平

生物来源

Saccharomyces cerevisiae

产品线

BioReagent

方案

≥99%

表单

powder

技术

cell culture | insect: suitable
cell culture | mammalian: suitable

溶解性

H2O: 50 mg/mL

SMILES字符串

[H]O[H].[H]O[H].OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H22O11.2H2O/c13-1-3-5(15)7(17)9(19)11(21-3)23-12-10(20)8(18)6(16)4(2-14)22-12;;/h3-20H,1-2H2;2*1H2/t3-,4-,5-,6-,7+,8+,9-,10-,11-,12-;;/m1../s1

InChI key

DPVHGFAJLZWDOC-PVXXTIHASA-N

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一般描述

海藻糖是一种自然界普遍存在的非还原性二糖。它不仅可以用作食品防腐剂,还可以保留酶、疫苗、细胞和许多其他分子。[1]海藻糖在无脊椎动物中以血淋巴糖的形式存在。[2]

应用

D-(+)-海藻糖二水合物可用于在慢速冷冻法中冷冻兔卵母细胞。[2]它可用于体外成熟卵母细胞的固体表面玻璃化(SSV)。[3][4]它在多种细胞冷冻培养基中适合用作冷冻保护剂。

生化/生理作用

海藻糖对于植物的胚发生、碳代谢、光合作用和开花至关重要。它主要存在于支持脱水生物(无水生活)的物种中。[1]它可作为微生物中的碳水化合物储备,并保护它们免受不利条件的影响,[5]如热、冷、脱水、干燥和氧化。[2]海藻糖可以作为影响蛋白质折叠的化学伴侣。它可在体外防止淀粉样蛋白的产生和 β-积聚。在亨廷顿蛋白聚集中也观察到了海藻糖的类似作用。因此,它可以作为阿尔茨海默氏′病和亨廷顿′病的保护剂。[2]

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Trehalose metabolism: from osmoprotection to signaling
Iturriaga G, et al.
International Journal of Molecular Sciences, 10(9), 3793-3810 (2009)
Developmental competence of in vitro-fertilized porcine oocytes after in vitro maturation and solid surface vitrification: effect of cryopreservation on oocyte antioxidative system and cell cycle stage
Somfai T, et al.
Cryobiology, 55(2), 115-126 (2007)
Production of good-quality porcine blastocysts by in vitro fertilization of follicular oocytes vitrified at the germinal vesicle stage
Somfai T, et al.
Theriogenology, 73, 147-156 (2010)
Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions.
El-Bashiti T, et al.
Plant Science, 169(1), 47-54 (2005)
Methodios Ximerakis et al.
Nature neuroscience, 22(10), 1696-1708 (2019-09-26)
The mammalian brain is complex, with multiple cell types performing a variety of diverse functions, but exactly how each cell type is affected in aging remains largely unknown. Here we performed a single-cell transcriptomic analysis of young and old mouse

Questions

1–5 of 5 Questions  
  1. We would like to dissolve trehalose in water in bulk and store it. Does dissolved trehalose loose its stability after an extended storage time? Which storage temperature would you recommend?

    1 answer
    1. The solution stability and storage of this product has not been tested, however other commercial sources offer 1M Trehalose solutions that are stored at ambient temperature or refrigerated (filter-sterilized). Trehalose is added to several of less stable protein preparations as a carrier and/or cryoprotectant. An example would be EGFR, E2645. This lyophilized product contains 10% Trehalose. Upon reconstitution the product can be stored for up to 1 year at -70 deg C. Given the information above, Trehalose may be quite stable in solution.

      Helpful?

  2. Can solutions of Product T0167, D-(+)-Trehalose dihydrate, be autoclaved?

    1 answer
    1. Do not autoclave. It is best to sterile filter solutions.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Can Product T0167, D-(+)-Trehalose dihydrate, be used as a substitute for protein in a freezing media?

    1 answer
    1. Yes, it should work as a substitute for protein in a freezing media.  It is commonly used at a final concentration of 5% (50 mg/mL).  Alternatively, one could use methylcellulose.

      Helpful?

  5. Do we have information of any fluorescent impurities in Product T0167, D-(+)-Trehalose dihydrate?

    1 answer
    1. This product is not tested for the presence or absence of fluorescent impurities.

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