If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
T1503
Trizma ® 碱
≥99.9% (titration), crystalline, primary standard, aminopeptidase substrate
别名:
2-氨基-2-(羟甲基)-1,3-丙二醇, THAM, Tris 碱, 三羟甲基氨基甲烷, 氨基丁三醇
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About This Item
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产品名称
Trizma ® 碱, Primary Standard and Buffer, ≥99.9% (titration), crystalline
品質等級
描述
aminopeptidase substrate
化驗
≥99.9% (titration)
形狀
crystalline
儲存條件
dry at room temperature
技術
ELISA: suitable
protein extraction: suitable
顏色
white
pH值
10.5-12
有用的pH值範圍
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
溶解度
methanol: soluble 26 mg/mL at 25 °C
ethylene glycol: soluble 79.1 mg/mL at 25 °C
water: soluble (678 g/l at 20 °C)
吸收
≤0.05 at 290 nm at 40%
適合性
suitable for Western blot
suitable for electrophoresis
應用
cell analysis
diagnostic assay manufacturing
life science and biopharma
SMILES 字串
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI 密鑰
LENZDBCJOHFCAS-UHFFFAOYSA-N
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一般說明
Tris 碱可能会用作碱度标准品、独立地作为缓冲剂以及作为混合缓冲剂配方（包括 Tris-EDTA (TE) 缓冲液、TAE 缓冲液、TBE 缓冲液等）中的关键成分。其属性包括纯度、基本稳定性和相对不吸湿性，使其成为实验室环境中的可靠选择。在这些环境中，Tris 碱对于制备与生物体液相容的缓冲液是必不可少的，并可作为标准 pH 溶液。它有利于各种实验室程序，例如乳酸脱氢酶测定、原位杂交和从细胞中提取蛋白质。Tris 碱的多功能性扩展到细胞生物学、生物化学和蛋白质研究，对涉及细胞膜通透性和缓冲液制备的研究做出了重大贡献。
應用
特點和優勢
- 在 pH 值 7 - 9 范围内有效缓冲，pKa 8.1 (25 °C)
- 经过测试，确认重金属污染水平较低，确保适合各种应用
- 可用于细胞生物学和生化研究
其他說明
对于精确的应用，使用经过精心校准的 pH 计和玻璃/甘汞组合电极。
法律資訊
也與該產品經常一起購買
同類產品
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Gloves
历史批次信息供参考:
分析证书(COA)
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实验方案
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
在测定羧肽酶A活性时，使用马尿酸-L-苯丙氨酸在254nm处进行连续分光光度法测定。羧肽酶A水解C末端残基上的肽键。
Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.
To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Is this product explosive in nature?
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As per the SDS of T1503: https://www.sigmaaldrich.com/sds/sigma/t1503?userType=anonymous, no GHS pictogram for explosive materials has been listed in Section 2, indicating it is not explosive in nature.
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Can I autoclave solutions that contain Product T1503, Trizma® base?
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Yes, Trizma buffered solutions may be autoclaved.
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Can Product T1503, Trizma® base be used as a standard?
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Trizma base meets the requirements for a primary basimetric standard.
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What is the pH of Trizma® base, T1503?
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The pH of a 0.1 M aqueous solution of Trizma base will have a pH of approximately 10.4.
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What type of electrode should I use to measure the pH of Trizma® base T1503?
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We recommend the use of glass-calomel electrodes for use with Trizma base solutions through a pH range of 0-12 and a temperature range of -5 °C to 80 °C. Silver/silver chloride reference electrodes are not recommended for use with Trizma solutions that contain proteins.
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How can I use T1503 to make a Trizma® buffered solution?
1 answer-
Trizma base may be used directly in preparing a buffer by adjusting the pH with a 0.1 to 1.0 M HCl or blended with Trizma hydrochloride.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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