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Fluorescent Dye Wavelength Index

Fluorescent dyes are used to tag proteins, nucleic acids, and other biomolecules for use in life science applications, including fluorescence microscopy, flow cytometry, fluorescence in situ hybridization (FISH), receptor binding assays, and enzyme assays. Using the right combination of fluorescent-labeled biomolecules can enable multiple targets to be visualized simultaneously. Fluorescent dyes are optimized for excitation and detection in the visible light to near infrared spectrum. The choice of fluorochrome to use is determined by its excitation and emission wavelengths and the application.

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Product No.	Product Name	Solvent	λ Ex Max	λ Em Max	Additional Conditions for Fluorescence	λ Ex Max	λ Em Max
01673	Acriflavine	EtOH	463	490
06627	2-Aminoacridone	pH 8.0	420	538
08445	7-Amino-4-methyl-3-coumarinylacetic acid	MeOH	350	433
08658	8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt	pH 7.0	356	512
09341	8-Aminopyrene-1,3,6-trisulfonic acid trisodium salt	pH 7.4	420	500
25102	9-(Chloromethyl)anthracene				Carboxylic acid adducts	365	412
10417	8-Anilino-1-naphthalenesulfonic acid ammonium salt	MeOH	372	480	Protein adduct	372	480
10580	Anthracene	CH₃CN	251	401
10609	9-Anthracenecarbonyl cyanide	MeOH	361	460	Hydroxyl compound adduct with ethanol	361	451
75068	9,10-Anthracenediyl-bis(methylene)dimalonic acid	pH 7.0	380	407
89313	Atto 390		390	475
89204	Atto 390 NHS ester		389	471
16805	Atto 425-NHS ester		437	483
50712	Atto 465
55607	Atto 465 maleimide		452	502
53404	Atto 465-NHS ester		449	503
41051	Atto 488		504	521
28562	Atto 488 maleimide		501	523
41698	Atto 488-NHS ester		498	520
16951	Atto 495		499	535
41022	Atto 495 maleimide		500	524
00379	Atto 495-NHS ester		499	524
77810	Atto 520 NHS ester	H₂O	520	542	EtOH	525	547
06699	Atto 532		533	560
68499	Atto 532 maleinimide		532	553
88793	Atto 532 NHS ester		530	551
40592	Atto 540 Q
62453	Atto 540 Q maleimide
61683	Atto 540 Q NHS ester
42424	Atto 550		554	574
30730	Atto 550 maleimide		554	576
92835	Atto 550 NHS ester		539	575
75784	Atto 565	H₂O	561	585	EtOH	566	590
72464	Atto 565 NHS ester	H₂O	561	585	EtOH	566	590
03722	Atto 580 Q
68152	Atto 580 Q maleimide
70425	Atto 590	H₂O	598	634	EtOH	598	634
79636	Atto 590 NHS ester	H₂O	598	634	EtOH	598	634
08637	Atto 594		601	627
08717	Atto 594 maleimide		601	627
08741	Atto 594 NHS ester		597	625
78493	Atto 610	H₂O	605	646	EtOH	616	630
78493	Atto 610		610	635
41061	Atto 610-maleimide		610	637
93259	Atto 610 N-succinimidyl ester	H₂O	605	646	EtOH	616	630
93259	Atto 610 N-succinimidyl ester		610	635
04327	Atto 612 Q
50332	Atto 612 Q maleimide
53988	Atto 612 Q NHS ester
92716	Atto 620		617	641
67351	Atto 620 NHS ester		620	641
18620	Atto 633
01464	Atto 633 NHS ester		630	650
08968	Atto 635		635	656
97875	Atto 647		643	665
41784	Atto 647 maleimide		647	669
04507	Atto 647N		644	667
07376	Atto 647 NHS ester		643	662
05316	Atto 647N maleimide		644	669
18373	Atto 647N NHS ester		647	661
93711	Atto 655	H₂O	665	690	EtOH	655	680
76245	Atto 655 NHS ester	H₂O	665	690	EtOH	655	680
75999	Atto 680 NHS ester	H₂O	680	702	EtOH	675	699
30674	Atto 700		681	714
50611	Atto 700 maleimide		645	715
16986	Atto 700 NHS-ester		681	713
47156	Atto 725		725	752
93725	Atto 725 NHS ester		725	753
91394	Atto 740		740	764
14448	2-(4-Biphenylyl)-6-phenylbenzoxazole	H₂O		425	CH3CN, MeOH		390
14510	1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid	pH 8.0	287	363	with calcium	274	363
14513	1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrasodium salt	pH 8.0	254	363	with calcium	274	363
14530	bisBenzimide H 33258	pH 7.0	338	505	bound to DNA	356	465
14533	bisBenzimide H 33342 trihydrochloride	pH 7.0	340	510	bound to DNA	355	465
14562	2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester				Reaction product	482	528
15025	meso-1,2-Bis(4-methoxyphenyl)ethylenediamine				Saccharide adducts	340	460
15090	1,4-Bis(2-methylstyryl)benzene	Cyclohexane		419	Heptane		416
69898	Bromobimane	pH 7.0	398		Thiol adduct	390	478
72301	9-(2-Carboxy-2-cyanovinyl)julolidine	pH 8.0	433	500
21882	5(6)-Carboxy-2',7'-dichlorofluorescein	pH 8.0	504	529
21884	5(6)-Carboxy-2',7'-dichlorofluorescein diacetate				Reaction product	470	504
86826	5-Carboxyfluorescein	pH 8.0	492	517
21877	5(6)-Carboxyfluorescein	pH > 8.0	490	520
21879	5(6)-Carboxyfluorescein diacetate				hydrolyzed	492	517
21888	5(6)-Carboxyfluorescein diacetate N-succinimidyl ester	DMF	<300		hydrolyzed	492	517
21878	5(6)-Carboxyfluorescein N-hydroxysuccinimide ester	pH 9.0	495	519
92846	5-Carboxyfluorescein N-succinimidyl ester	pH 8.0	492	517
21965	5(6)-Carboxy-X-rhodamine	MeOH	568	595
41969	5-Carboxy-X-rhodamine N-succinimidyl ester	pH 7.0	575	600
21953	5(6)-Carboxytetramethylrhodamine	MeOH	541	566
55222	6-Carboxytetramethylrhodamine	pH 8.0	543	575
53048	5-Carboxy-tetramethylrhodamine N-succinimidyl ester	pH 7.0	543	575
21955	5(6)-Carboxytetramethylrhodamine N-succinimidyl ester	MeOH	540	565
88997	6-Carboxy-tetramethylrhodamine N-succinimidyl ester	pH 7.0	543	575
25455	4-Chloro-7-nitrobenzofurazan	MeOH	336		Aliphatic amine adducts	475	540
30423	3-(Dansylamino)phenylboronic acid	MeOH	337	517	Protein adduct	337	490
30432	Dansylcadaverine	MeOH	335	518	Reaction product	338	500
39220	Dansyl chloride	CHCl₃	372		Butylamine adduct	337	492
30434	Dansylhydrazine	MeOH	336	534	Saccharide adducts	336	487
32670	4',6-Diamidino-2-phenylindole dihydrochloride	H₂O			bound to DNA	358	461
34025	Dibromobimane	MeOH	391		Thiol adduct	391	480
72335	9-(2,2-Dicyanovinyl)julolidine	pH 8.0	433	500
36798	7-(Diethylamino)coumarin-3-carbohydrazide	MeOH	420	468	Carboxylic acid adduct	415	480
31755	7-(Diethylamino)coumarin-3-carbonyl azide	MeOH	432	480
36799	7-(Diethylamino)coumarin-3-carboxylic acid	pH 9.0	409	473
36801	7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester	MeOH	432	472
37150	3,3'-Diheptylthiacarbocyanine iodide	MeOH	562	575
37291	Dihydroethidium	MeCN	355	420	bound to DNA	358	461
37920	6,8-Dihydroxy-1,3-pyrenedisulfonic acid disodium salt	pH 4.0	405	465	pH 8.0	458	500
39235	11-[5-(Dimethylamino)-1-naphthalenesulfonylamino]undecanoic acid	MeOH	335	519
39255	4-Dimethylamino-4'-nitrostilbene	CH2Cl₂	500	700
41525	N,N-Dimethyl-6-propionyl-2-naphthylamine	MeOH	361	498
41785	Dimidium bromide	pH 7.6, low ionic strength	480		Nucleic acid adducts, Tris pH 8.0	306	612
42364	1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate	MeOH	550	565
43608	3,3'-Dipropylthiadicarbocyanine iodide	MeOH	651	675
40227	6-Dodecanoyl-N,N-dimethyl-2-naphthylamine	MeOH	364	497
44583	Doxorubicin hydrochloride	EtOH	470	585
45245	Eosin 5-isothiocyanate	pH 9.0	521	544
46067	Ethidium bromide solution	H₂O	480	620	bound to DNA	518	605
46043	Ethidium homodimer	H₂O	493		bound to DNA	528	617
46123	1-(Ethoxycarbonylmethyl)-6-methoxyquinolinium bromide	H₂O	355	460
46125	8-Ethoxy-N,N,N',N',N'',N''-hexamethylpyrene-1,3,6-trisulfonamide				Reaction product	380	520
46393	Fluo-3	pH > 6.0	503		with calcium	506	526
46917	9-Fluorenylmethyl carbazate	MeOH	264	309	Saccharide adducts	270	320
46955	Fluorescein	pH 9.0	490	514
46960	Fluorescein sodium salt	pH 9.0	490	514
46935	6-[Fluorescein-5(6)-carboxamido]hexanoic acid	pH 9.0	480	525
46940	6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester	pH 8.2	491	515
46943	Fluorescein dilaurate				Reaction product	490	514
46950	Fluorescein 5(6)-isothiocyanate	pH 9.0	495	520
46946	Fluorescein isothiocyanate–dextran 150000	pH 8.0	490	520
46945	Fluorescein isothiocyanate–dextran 70000	pH 8.0	490	520
46947	Fluorescein isothiocyanate – Dextran 500000-Conjugate	pH 8.0	490	520
46985	Fluorescein-5-thiosemicarbazide	pH 9.0	492	516
55817	Fluorescent red 630	pH 7.0	620	650
47140	4-Fluoro-7-nitrobenzofurazan	EtOH	470	528	Amino acid adducts	470	530
47614	Fluram^®	pH 9.0	234		Protein adduct	334	455
52335	N-Hexadecylpyrene-1-sulfonamide	MeOH	350	400
55155	7-Hydroxycoumarin-3-carboxylic acid	pH 4.0	342	447	pH 9.0	386	448
55156	7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester	MeOH	418	447
55634	7-Hydroxy-4-methyl-3-coumarinylacetic acid	pH 10.0	360	445
55627	7-Hydroxy-4-methyl-2(1H)-quinolone	pH 5.0	321	357	pH 9.0	351	438
56059	7-Hydroxy-N-octadecylcoumarin-3-carboxamide	CHCl₃/DMSO	359	406
57180	Indo 1	100 mM KCl	349	482	with calcium	331	398
64951	7-Methoxycoumarin	CHCl₃	340	389	Reaction product	360	449
64948	7-Methoxycoumarin-3-carboxylic acid	MeOH	360	415
64958	2-Methoxy-2,4-diphenyl-3(2H)-furanone				Amine adducts	390	480
69025	1-Methylpyrene	CHCl₃	340	486
69587	2'-(4-Methylumbelliferyl)-a- D -N-acetylneuraminic acid sodium salt hydrate	pH 7.0	315	374	Reaction product	360	449
69585	4-Methylumbelliferyl N-acetyl-ß- D -glucosaminide dihydrate	pH 10.0	317		Reaction product	360	449
19362	4-Methylumbelliferyl butyrate	MeOH	312		Reaction product	360	449
69591	4-Methylumbelliferyl a- D -glucopyranoside	pH 9.0	317	374	Reaction product	360	449
69602	4-Methylumbelliferyl-ß- D -glucuronide hydrate	pH 9.0	316	375	Reaction product	360	449
51010	4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate	pH 6.0	323	464	Reaction product	360	449
75164	4-Methylumbelliferyl oleate	MeOH	312		Reaction product	360	449
69899	Monochlorobimane	MeOH	380	461	Thiol adduct	380	480
70420	Naphthofluorescein	pH 10.0	594	663
70450	2-Naphthol	0.1 M NaOH	320	410
70448	2-Naphthol	0.1 M NaOH	320	410
70684	1-(2-Naphthoyl)imidazole	H₂O	230	362	Aldite adducts	234	374
15167	NIR-797 isothiocyanate	MeOH	668	798
72963	12-(7-Nitrobenzofurazan-4-ylamino)dodecanoic acid	MeOH	467	539
72964	6-(7-Nitrobenzofurazan-4-ylamino)hexanoic acid	MeOH	467	539
74758	8-Octadecyloxypyrene-1,3,6-trisulfonic acid trisodium salt	H₂O	404	434
74875	8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt	H₂O	384	409	Reaction product	460	520
75926	Oxonol VI	MeOH	599	634
76275	PBFI-AM	MeOH	369		with Na	334	525
77490	Phenanthridine	DMSO	265	415
79279	3-Phenylumbelliferone	pH 3.0	338	463	pH 10.0	383	472
79760	Phthaldialdehyde	pH 7.0	257		Thiol adduct	334	455
81845	Propidium iodide	pH 7.2	482	608	bound to DNA	540	608
82648	Pyrene	n-Pentane	350	392
82660	1-Pyrenedecanoic acid	MeOH	341	377
82663	1-Pyrenedodecanoic acid	MeOH	341	377
82657	1-Pyrenesulfonic acid sodium salt	MeOH	344	376
08520	Quin-2				with calcium	332	493
22620	Quinine	0.5 M Sulfuric acid	347	448
22640	Quinine hemisulfate salt monohydrate	H₂O	343	453
22630	Quinine hydrochloride dihydrate	H₂O	322	450
83636	Resorufin acetate	DMSO	540	590	Reaction product	571	585
83637	Resorufin butyrate	DMSO	540	590	Reaction product	571	585
46121	Resorufin ethyl ether	MeOH	464
69125	Resorufin methyl ether	MeOH	463
83689	Rhodamine B	Acidic EtOH	554	627
83697	Rhodamine 6G	MeOH	528	551
83694	Rhodamine 101	MeOH	565	589
83695	Rhodamine 110 chloride	MeOH	499	521
83701	Rhodamine 800	MeOH	528	547
83685	Rhodamine B octadecyl ester perchlorate	MeOH	554	578
17866	Streptavidin-CryptoFluor™ Crimson	pH 7.2	585	658
86186	Sulforhodamine B acid chloride	H₂O	565	586
86470	p-Terphenyl	EtOH	277
87917	Tetramethylrhodamine ethyl ester perchlorate	MeOH	549	574
87918	Tetramethylrhodamine isothiocyanate mixed isomers	MeOH	543	571
88071	meso-Tetraphenylporphyrin	Toluene	645	651
43060	N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl)phenylammonium p-toluenesulfonate	MeOH	355	450
93979	Umbelliferone	95% EtOH	325	441

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