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Sigma-Aldrich

Atto 550 maleimide

BioReagent, suitable for fluorescence, ≥90.0% (degree of coupling)

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About This Item

UNSPSC Code:
12352111
NACRES:
NA.32

product line

BioReagent

Assay

≥90.0% (HPLC)
≥90.0% (degree of coupling)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0,1% trifluoroacetic acid)

UV absorption

λ: 552-558 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550 maleimide is suitable for fluorescence labeling of thiol/SH containing molecules.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Frauke Beilstein et al.
Cell reports, 32(7), 108042-108042 (2020-08-20)
VSV fusion machinery, like that of many other enveloped viruses, is triggered at low pH in endosomes after virion endocytosis. It was suggested that some histidines could play the role of pH-sensitive switches. By mutating histidine residues H22, H60, H132
Rula Masoud et al.
ACS nano, 6(7), 6272-6283 (2012-06-06)
We present a test case example of a detailed single-molecule fluorescence study of one of the most sophisticated and complex DNA devices introduced to date, a recently published autonomous bipedal DNA motor. We used the diffusion-based single-molecule Förster resonance energy
Rahul Roy et al.
Nature methods, 5(6), 507-516 (2008-05-31)
Single-molecule fluorescence resonance energy transfer (smFRET) is one of the most general and adaptable single-molecule techniques. Despite the explosive growth in the application of smFRET to answer biological questions in the last decade, the technique has been practiced mostly by
A molecular toolkit for population genetic investigations of the ash dieback pathogen Hymenoscyphus pseudoalbidus.
Gross, A., et al.
Forest Pathology, 42, 252-264 (2012)

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