Atto 740 is a new infrared label with high molecular absorption (120.000) and sufficient stoke′s shift between excitation and emission maximum. Atto 740 is characterized by high photostability and thermal stability. It is moderately soluble in water. ATTO 740 is a pH sensitive product. While practically stable at pH 7.4 (PBS Buffer), it slowly degrades at higher pH. After 7 hours at pH 8.25 (0.2 M sodiumbicarbonate buffer) 85% of the dye is retained.
Journal of biophotonics, 12(3), e201800185-e201800185 (2018-11-14)
We introduce a simple new approach for time-resolved multiplexed analysis of complex systems using near-infrared (NIR) dyes, applicable to in vitro and in vivo studies. We show that fast and precise in vitro quantification of NIR fluorophores' short (subnanosecond) lifetime
Journal of biomedical optics, 15(4), 046011-046011 (2010-08-31)
Near-infrared (NIR) fluorescence tomography of multiple fluorophores has previously been limited by the bandwidth of the NIR spectral regime and the broad emission spectra of most NIR fluorophores. We describe in vivo tomography of three spectrally overlapping fluorophores using fluorescence
The journal of physical chemistry. B, 115(29), 9320-9326 (2011-06-22)
Fluorescence correlation spectroscopy (FCS) can be coupled with Förster resonance energy transfer (FRET) to detect intramolecular dynamics of proteins on the microsecond time scale. Here we describe application of FRET-FCS to detect fluctuations within the N-terminal and C-terminal domains of
Tip-enhanced single molecule fluorescence near-field microscopy in aqueous environment.
Block copolymer-based vesicles have recently garnered a great deal of interest as nanoplatforms for drug delivery and molecular imaging applications due to their unique structural properties. These nanovesicles have been shown to direct their cargo to disease sites either through
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