X4626

XTT sodium salt

powder, BioReagent, suitable for cell culture

• Synonim(y): 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt
• Wzór empiryczny (zapis Hilla): C₂₂H₁₆N₇NaO₁₃S₂
• Numer CAS: 111072-31-2
• Masa cząsteczkowa: 673.52
• Numer MDL: MFCD00083517
• Kod UNSPSC: 12352207
• Identyfikator substancji w PubChem: 24902150
• NACRES: NA.75

Właściwości

• linia produktu: BioReagent
• Próba: ≥90%
• Postać: powder
• warunki przechowywania: dry at room temperature
• metody: cell culture | mammalian: suitable; protein quantification: suitable
• Zastosowanie: detection
• metoda wykrywania: colorimetric
• temp. przechowywania: 2-8°C
• ciąg SMILES: [Na+].COc1cc(c(cc1-n2nc(n[n+]2-c3cc(c(cc3OC)[N+]([O-])=O)S([O-])(=O)=O)C(=O)Nc4ccccc4)S([O-])(=O)=O)[N+]([O-])=O
• InChI: 1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);/q;+1/p-1
• Klucz InChI: JACYMBNQPPWQML-UHFFFAOYSA-M

Opis

Opis ogólny

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.

Zastosowanie

XTT sodium salt has been used to assess cell viability.

XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines. The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported.

Działania biochem./fizjol.

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.

Zasada

In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H242
• Zwroty wskazujące środki ostrożności: P210 - P234 - P235 - P240 - P370 + P378 - P403
• Klasyfikacja zagrożeń: Self-react. C
• Zagrożenia dodatkowe: EUH044
• Kod klasy składowania: 4.1A - Other explosive hazardous materials
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)