V4888

Anti-VSV-G antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonim(y): Anti-Vesicular Stomatitis Virus glycoprotein
• Numer MDL: MFCD00164761
• Kod UNSPSC: 12352203
• NACRES: NA.56

Właściwości

• pochodzenie biologiczne: rabbit
• białko sprzężone: unconjugated
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• Formularz: buffered aqueous solution
• reaktywność gatunkowa: mammals
• stężenie: ~1.0 mg/mL
• metody: immunoprecipitation (IP): 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates (tagged fusion proteins from transfected mammalian cell lysates); indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells; western blot: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates
• Warunki transportu: dry ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

The Anti-VSV-G rabbit antibody reacts with amino- and carboxyl-terminal VSV-G tagged fusion proteins.

Vesicular stomatitis viruses (VSV) belongs to the Vesiculovirus genus of the Rhabdoviridae family.[1] Vesicular stomatitis virus G (VSV G) protein is considered to be the early recognized type III viral fusion protein.[2]

Immunogen

synthetic peptide corresponding to amino acids 497-511 (N-Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys-C) of the Vesicular Stomatitis Virus glycoprotein (VSV-G), conjugated to maleimide activated KLH via a cysteine residue added at the carboxyl terminus. The antibody recognizes the VSV-G tag at both the C-terminus and N-terminus.

Zastosowanie

Anti-VSV-G antibody produced in rabbit has been used in western blotting[3][4] and immunoprecipitation.[5][6]

Antibody is suitable for immunoblotting, immunoprecipitation, and indirect immunofluorescence.
immunocytochemistry.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Western Blotting (1 paper)
Western blotting following immunoprecipitation (1 paper)

Działania biochem./fizjol.

Vesicular stomatitis virus G (VSV G) is capable of stimulating cell-cell fusion upon low pH stimulation. The transmembrane region of VSV G protein is highly essential for the virus transition from the hemi-fusion to full fusion state.[2]

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable