SAB4200489

Anti-Occludin (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonim(y): Anti-OCLN
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• białko sprzężone: unconjugated
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• Formularz: buffered aqueous solution
• masa cząsteczkowa: antigen ~65 kDa
• reaktywność gatunkowa: human, dog
• stężenie: ~1.0 mg/mL
• metody: immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon.; indirect immunofluorescence: 1-2 μg/mL using MDCK cells; western blot: 1.5-3.0 μg/mL using extracts of Caco-2 cells
• numer dostępu UniProt: Q16625
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... OCLN(100506658)

Opis

Opis ogólny

Occludin (OCLN) is an integral membrane protein, encoded by the gene mapped to human chromosome 5q13.2. OCLN has a molecular weight of about 65kDa. It possesses four transmembrane domains including a long COOH-terminal cytoplasmic domain, a short NH2-terminal cytoplasmic domain, two extracellular loops and one intracellular turn. It is specifically localized at tight junctions (TJ).

Immunogen

a synthetic peptide corresponding to a sequence at the C-terminal region of human occludin, conjugated to KLH. The corresponding sequence is highly conserved in rat (89% sequence identity) and in mouse (84% sequence identity) occludin.

Zastosowanie

Anti-Occludin (C-terminal) antibody produced in rabbit has been used in immunohistochemistry.

Działania biochem./fizjol.

Occludin (OCLN), along with tight junction (TJ)-associated peripheral membrane proteins plays a vital role in the formation and regulation of TJ. The encoded protein facilitates the translocation of p85a (regulatory subunit of phosphoinositide 3-kinase) to TJs to regulate actin organization after oxidative stress. OCLN is also implicated in cell migration. Mutation in the gene leads to the development of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG).

Postać fizyczna

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable