AB2272

Anti-ZO-1 Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): tight junction protein 1, zonula occludens 1 protein, Zona occludens protein 1, Zonula occludens protein 1, zona occludens 1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• reaktywność gatunkowa (przewidywana na podstawie homologii): mouse (based on 100% sequence homology)
• opakowanie: antibody small pack of 25 μg
• metody: immunohistochemistry: suitable (paraffin); western blot: suitable
• numer dostępu NCBI: NP_003248
• numer dostępu UniProt: Q07157
• Warunki transportu: ambient; wet ice
• temp. przechowywania: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... TJP1(7082)

Opis

Opis ogólny

Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.

Specyficzność

Other homologies: Rat ( 89% sequence homology).

Immunogen

KLH-conjugated linear peptide corresponding to human ZO-1.

Zastosowanie

Detect ZO-1 using this Anti-ZO-1 Antibody validated for use in WB, IH(P).

Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected ZO-1 in normal human testis tissue.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Jakość

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected ZO-1 on 10 µg of HepG2 cell lysate.

Opis wartości docelowych

~260 kDa observed

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
HepG2 cell lysate

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable