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RECOMT

Sigma-Aldrich

Thrombin CleanCleave™ Kit

Synonim(y):

Thrombin Cleavage Kit

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About This Item

Kod UNSPSC:
12352200
NACRES:
NA.56

Postać

suspension

Poziom jakości

200

masa cząsteczkowa

37 kDa

metody

protein purification: suitable

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Opis ogólny

The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins

Zastosowanie

For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.

Cechy i korzyści

  • Fast, efficient cleavage in as little as 2 hours
  • Thrombin is covalently bound to agarose for easy removal.
  • The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
  • Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
  • Thrombin-agarose is reusable.

Ilość

200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.

Informacje prawne

CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
This page may contain text that has been machine translated.

Tylko elementy zestawu

Numer produktu
Opis
  • Thrombin Cleavage Buffer, 10× 10 mL

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Kod klasy składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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