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Merck

R1383

Sigma-Aldrich

RPMI-1640 Medium

With ʟ-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

Synonim(y):

Roswell Park Memorial Institute 1640 medium

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10 X 1 L
748,00 zł

748,00 zł


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10 X 1 L
748,00 zł

About This Item

Numer MDL:
Kod UNSPSC:
12352207
NACRES:
NA.75

748,00 zł


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Nazwa produktu

RPMI-1640 Medium, With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

Formularz

powder

metody

cell culture | mammalian: suitable

komponenty

L-glutamine: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Opis ogólny

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Zastosowanie

RPMI-1640 Medium has been used as a cell culture medium to culture human MCF-7 breast cancer cells[1] and human castrate-resistant prostate cancer (CRPC) cell lines for various cell culture experiments. It has also been used to culture human islets to assess the oxidative stress response in primary human islets.

Ilość

Formulated to contain 8.4 grams of powder per liter of medium.

Rekonstytucja

Supplement with 2.0 g/L sodium bicarbonate.
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Kod klasy składowania

13 - Non Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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