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Merck

O1391

Sigma-Aldrich

Oil Red O solution

0.5% in isopropanol

Synonim(y):

Solvent Red 27, Sudan Red 5B

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250 ML
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488,00 zł

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250 ML
263,00 zł
500 ML
488,00 zł

About This Item

Wzór empiryczny (zapis Hilla):
C26H24N4O
Numer CAS:
Masa cząsteczkowa:
408.49
Numer WE:
Kod UNSPSC:
12171500
NACRES:
NA.47

263,00 zł


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Formularz

liquid

stężenie

0.5% in isopropanol

metody

microbe id | staining: suitable

kolor

dark red

εmax

0.20-0.50 at 510-517 nm

Zastosowanie

diagnostic assay manufacturing
hematology
histology

temp. przechowywania

room temp

ciąg SMILES

N(\N=C3\c4c(cccc4)C=CC\3=O)c1c(cc(c(c1)C)N=Nc2c(ccc(c2)C)C)C

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-

Klucz InChI

HJAYEODIXUYVIC-LTFAAIOASA-N

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Opis ogólny

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.[1][2]

Zastosowanie

Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.
Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts.[3][4][5]
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Piktogramy

FlameExclamation mark

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Organy docelowe

Central nervous system

Kod klasy składowania

3 - Flammable liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

53.6 °F - closed cup

Temperatura zapłonu (°C)

12.0 °C - closed cup


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Hepatic Peroxisome Proliferator-Activated Receptor Gamma Signaling Contributes to Alcohol-Induced Hepatic Steatosis and Inflammation in Mice.
Zhang W, et al.
Alcoholism, Clinical and Experimental Research, 40(5), 988-999 (2016)
Directly reprogramming fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by defined factors.
Wu W, et al.
Experimental and Therapeutic Medicine, 13(6), 2685-2690 (2017)
LWW Doody's all reviewed collection, McClatchey: Clinical Laboratory Medicine
Clinical Laboratory Medicine (2002)
Sulforaphane inhibits mammary adipogenesis by targeting adipose mesenchymal stem cells.
Li Q, et al.
Breast Cancer Research and Treatment, 141(2), 317-324 (2013)
Pablo Sanchez Bosch et al.
Developmental cell, 51(6), 787-803 (2019-11-19)
The use of adult Drosophila melanogaster as a model for hematopoiesis or organismal immunity has been debated. Addressing this question, we identify an extensive reservoir of blood cells (hemocytes) at the respiratory epithelia (tracheal air sacs) of the thorax and

Produkty

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Protokoły

Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.

Protokoły różnicowania osteogenezy, adipogenezy i chondrogenezy oraz pożywki do hodowli ludzkich mezenchymalnych komórek macierzystych. Poznaj ponad 350 produktów PromoCell na naszej stronie internetowej.

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Informacje na temat mezenchymy, w szczególności protokołów mezenchymalnych komórek macierzystych. Protokoły hodowli komórkowej krok po kroku do izolacji, ekspansji i różnicowania mezenchymalnych komórek macierzystych (MSC).

Questions

1–6 of 6 Questions  
  1. Hi! I'm wondering if OilRedO is light sensitive? Does the incubation need to take place in the dark? And could the staining of cells be compromized if the solution is not kept dark?

    1 answer
    1. There are no definitive references or citations confirming the light sensitivity of this material. However, this product is packaged in amber glass may be susceptible to light, particularly at UV wavelengths. In bright field microscopy slides must be viewed in subdued light. While this item is not tested for use in fluorescent, it has been used on Flow Cytometry and has an emission wavelength of approximately 600 nm. There are a number of publication indicating light sensitivity. See the link below to review on such example:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931440/

      Helpful?

  2. What are the differences between Oil Red O solution O1391 and OIL RED O staining solution 1.02419?

    1 answer
    1. There are not many differences between products O1391 and 102419. O1391 is available in 250 mL and 500 mL bottles, while 102419 is available only in a 250 ml bottle. The only practical difference between the two products is the solvents used to dissolve the Oil Red O. For O1391, the solvent used is isopropyl alcohol, while the solvents used for 102419 are acetone and ethyl alcohol. Both products use Oil Red O as the dye, and the directions for use call for diluting the product with water prior to use and then filtering. The results should be identical since both products use Oil Red O as the dye.

      Helpful?

  3. Should cells or tissues be fixed before staining with Oil Red O for fats or lipids?

    1 answer
    1. Yes, cells or tissues need to be fixed before staining with Oil Red O. It is important to avoid using fixatives that contain alcohol. Oil Red O staining can be performed on cultured cells and fresh frozen tissue. However, tissues should not be paraffin embedded and exposed to the alcohols and solvents necessary for paraffin processing tissue.

      Helpful?

  4. Hi I purchased Oil Red O solution – 0.5% in isopropanol, 01391 250mL, PCode-1003585017, Source, SLCP2956 for staining of whole mice aorta. I want to dilute it to 0.16% solution. What solution should I use for dilution? MilliQ water/methanol/isopropanol?

    1 answer
    1. This product can be further diluted using distilled water or aqueous buffer to reach the desired concentration. Note that solution diluted with water or aqueous buffers are less stable and should not be stored for extended periods.

      Helpful?

  5. Can I use this solution for stain frozen sections ? I thought that I bought oil red in propylene glycol. What is the difference ?

    1 answer
    1. This O1391 is a 0.5% solution in isopropanol. Product O1516 is a 0.5% solution in propylene glycol. The protocol for either product is the same. There is some evidence in the literature that the propylene glycol solution performs better on PFPP samples, however this is not definitive and may depend on conditions and technique. Each is considered a stock concentration. Stock solutions can range from 0.2 - 0.5%. Depending on the specific application the stock solution will need to be further diluted by 40 - 60%. For example, in staining frozen sections, 30ml of the O1391 would be diluted with 20ml of distilled water to reach a working concentration. Note that solution diluted with water or aqueous buffers are less stable and should not be stored for extended periods.

      Please see the link below to review and article that may be helpful:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449473/pdf/10616_2004_Article_3903.pdf

      Helpful?

  6. How do you prepare the working solution using this reagent? or is it already a prepared working solution?

    1 answer
    1. This product is a 0.5% solution in isopropanol and is considered a stock concentration. Stock solution can range from 0.2 - 0.5%. Depending on the specific application the stock solution will need to be further diluted by 40 - 60%. For example, in staining frozen sections, 30ml of the O1391 would be diluted with 20ml of distilled water to reach a working concentration. Note that solution diluted with water or aqueous buffers are less stable and should not be stored for extended periods.

      Helpful?

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