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MAK107

LCAT Activity Assay Kit

Supplied by Roar Biomedical, Inc.

Synonim(y):

Lecithin:cholesterol acyltransferase activity assay kit

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Informacje o tej pozycji

UNSPSC Code:
12161503
EC Number:
200-659-6
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

application(s)

pharmaceutical

detection method

fluorometric

relevant disease(s)

cardiovascular diseases

storage temp.

−20°C

Gene Information

human ... LCAT(3931)

General description

The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.

Application

Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.
Suitable for measuring phospholipase activity of LCAT in plasma or serum.
LCAT Activity Assay Kit has been used for measuring lecithin-cholesterol acyl transferase activity in plasma samples.

Biochem/physiol Actions

The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λEx=390 nm/λEm=470 nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.

Features and Benefits

Compatible with high-throughput handling systems.

Analysis Note

Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.
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Tylko elementy zestawu

Numer produktu
Opis
  • Substrate Reagent .1 mL
  • READ Reagent 30 mL
  • LCAT Assay Buffer 20 mL

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Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Klasa składowania

3 - Flammable liquids

flash_point_f

49.5 °F

flash_point_c

9.7 °C

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–3 of 3 Questions  

  1. What substrate should be used to create the standard curve for this assay?

    1 answer

    1. Product No. MAK107 comes with a "Substrate Reagent" component which is used as the substrate in this assay.

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  2. Are products MAK107 and MAK108 compatible with mouse serum?

    1 answer

    1. Yes, but please be aware of possible sample hemolysis. Depending on the method of mouse sample collection, hemolysis may be common, which can interfere with fluorescence emission via color quenching.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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