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MAK044

Sigma-Aldrich

Free Fatty Acid Quantitation Kit

sufficient for 100 colorimetric or fluorometric tests

Synonim(y):

Free Fatty Acid Assay Kit

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About This Item

Kod UNSPSC:
12161503

zastosowanie

sufficient for 100 colorimetric or fluorometric tests

Zastosowanie

cosmetics
food and beverages

metoda wykrywania

colorimetric
fluorometric

powiązane choroby

endocrinological disorders, diabetes; cancer

temp. przechowywania

−20°C

Opis ogólny

Free fatty acids, also know as nonesterified fatty acids, circulate in the plasma bound to albumin. The majority of free fatty acids are derived from either dietary sources or are mobilized from adipose tissue. Plasma free fatty acids are elevated in many obesity-related disorders and may contribute to insulin resistance in peripheral tissues. Conditions such as sepsis and tumors producing lipoactive homones may also be associated with elevated free fatty acid levels.

Abnormalities in fatty acid metabolism causes fatty liver disease.[1] Accumulation of free fatty acid in myocytes leads to the risk of cardiac dysfunction.[2] Increased fatty acid β-oxidation is implicated in cardiac ischemia.[2]

Zastosowanie

Free Fatty Acid Quantitation Kit has been used in the following:
  • to measure lipid hydrolysis, by determining the free fatty acid in digested sample.[3]
  • to measure plasma and serum free fatty acids level.[4][5]
  • to quantify free fatty acids level in liver tissue.[6]

Przydatność

Suitable for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc.

Zasada

In this kit, the concentration of fatty acids (C8 and longer) is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the fatty acids present.
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Numer produktu
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Cennik

92210

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06693

Timestrip Plus™ -20 °C

zastąpiony przez

Numer produktu
Opis
Cennik

Piktogramy

Health hazard
GHS08

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H334

Zwroty wskazujące środki ostrożności

P261 - P284 - P501

Klasyfikacja zagrożeń

Resp. Sens. 1

Kod klasy składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

188.6 °F - closed cup

Temperatura zapłonu (°C)

87 °C - closed cup

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
8L11K06120
8A17K06120
8C21K06120
8E18K06120
8B15K06120

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Sarmistha Mukherjee et al.
Hepatology (Baltimore, Md.), 65(2), 616-630 (2016-11-04)
The regenerative capacity of the liver is essential for recovery from surgical resection or injuries induced by trauma or toxins. During liver regeneration, the concentration of nicotinamide adenine dinucleotide (NAD) falls, at least in part due to metabolic competition for
Association of Temperament and acute stress responsiveness with Productivity, Feed efficiency, and Methane emissions in Beef cattle: an Observational study
Llonch P, et al.
Frontiers in veterinary science, 3, 43-43 (2016)
Transgenic expression of fatty acid transport protein 1 in the heart causes lipotoxic cardiomyopathy.
Chiu H C, et al.
Circulation Research, 96(2), 225-233 (2005)
Lipid metabolism and liver inflammation. II. Fatty liver disease and fatty acid oxidation
Reddy J K and Sambasiva R M
American Journal of Physiology: Gastrointestinal and Liver Physiology, 290(5) (2006)
Kuifen Ma et al.
Iranian journal of basic medical sciences, 20(11), 1207-1212 (2018-01-05)
Arachidonic Acid/5-lipoxygenase (AA/5-LOX) pathway connects lipid metabolism and proinflammatory cytokine, which are both related to the development and progression of nonalcoholic fatty liver disease (NAFLD). Therefore, the present study was designed to investigate the role of AA/5-LOX pathway in progression
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Questions

1–9 of 9 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is there a step in the process where samples can be preserved for future analysis using the kit?

    1 answer

    1. Yes, samples can be preserved for future analysis using the kit by aliquoting and freezing them if needed for multiple uses. However, it's recommended to use fresh samples whenever possible to ensure accurate results. Dried lipids can be stored in 200 µl of Fatty Acid Assay Buffer at -80°C. In case of lipid precipitation, re-dissolve the lipids by sonication. However, the supplier does not specify the maximum duration for which the samples stored at -80°C will remain stable. Storing samples at -80°C may impact their stability, potentially resulting in lower than expected readings. Therefore, it is recommended to use fresh samples for this assay whenever possible to ensure accurate results.
      If frozen samples are used, they should be snap frozen in liquid nitrogen immediately after collection. These samples cannot undergo multiple freeze-thaw cycles and must be stored for relatively short periods. It's important to note that stability studies on different sample types have not been conducted, so there is no advice available regarding the timeframe for measuring frozen samples.

      Helpful?

  4. Can MAK044 quantify only albumin-bound free fatty acids (FFA), or does it also quantify unbound FFA?

    1 answer

    1. The kit specifically detects unbound free fatty acids in the samples and does not detect any albumin-bound free fatty acids.

      Helpful?

  5. What are the recommended storage instructions for the Enhancer reagent and unused Assay Buffer in the MAK044 Free Fatty Acid Quantitation Kit?

    1 answer

    1. The enhancer can be aliquoted and stored at -20°C, while the assay buffer can also be stored in aliquots at -20°C to avoid multiple freeze-thaw cycles.

      Helpful?

  6. I would like to quantify butyric acid in bacteria culture supernatant. Does this MAK044 Free Fatty Acid Quantitation Kit suitable or is there a kit you can recommend. Thank you. 

    1 answer

    1. Butyric acid is a short-chain fatty acid. This kit is suitable for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma, and other body fluids, food, growth media, etc. The Short Chain Fatty Acid Kit, prod number SBR0030, offers a potential analytical option. This kit is suitable for detection of several SCFAs, including Butyric, by HPLC, LC-MS, and GC-MS. See the link below to the technical bulleting should these methods be suitable:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/831/SBR00030bul.pdf

      Helpful?

  7. Can this kit be used to analyse NEFA in plasma using the Pentra C400 instrument?

    1 answer

    1. This kit is suitable for detecting nonesterified fatty acids in plasma (NEFA). However, suitability with Pentra C400 instrument has not been determined. This application must be determined by the end-user.

      Helpful?

  8. Can these kits be used with serum samples from mouse?

    1 answer

    1. Yes, this kit can be used on mouse serum samples. We generally recommend that fresh serum samples be used. Frozen samples can probably be used. However, the successful use of frozen samples depends on the efficiency of freezing them and the amount of time they have been frozen. If previously frozen serum samples are used, you may want to check the performance of the kit with fresh vs. frozen samples.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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