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MAK266

Triglyceride Quantification Colorimetric/Fluorometric Kit

sufficient for 100 colorimetric or fluorometric tests

1 of 3 reviewers received a sample product or took part in a promotion

Synonim(y):

TG Quantification Kit

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Informacje o tej pozycji

NACRES:
NA.84
UNSPSC Code:
12161503

storage condition

dry at room temperature

technique(s)

activity assay: suitable

input

serum
plasma

detection method

colorimetric, fluorometric

relevant disease(s)

neurological disorders; cardiovascular diseases

storage temp.

−20°C

General description

The new Triglyceride Quantification Kit, MAK564, is now available! Triglycerides (TG) are the main constituents of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as an energy source. TG is broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.

Application

Triglyceride Quantification Colorimetric/Fluorometric Kit has been used to measure triglyceride levels in:
  • human liver tissue samples and mouse models
  • cardiac tissue samples
  • cell lines
Suitable for use with serum, plasma, and other biological fluids, and tissue and cell culture samples.

Biochem/physiol Actions

The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In this assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidized to generate a colorimetric (570 nm)/fluorometric (lex = 535 nm/lem = 587 nm) product. The kit is sensitive to detect 2 pmole–10 nmole (2–10,000 mM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.
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pictograms

Health hazard
GHS08

signalword

Danger

Klasa składowania

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 2

Hazard Classifications

ED ENV 1 - Resp. Sens. 1

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Certyfikaty analizy (CoA)

Lot/Batch Number

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3 Reviews This action will navigate to reviews.

Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Can ethanol or isopropanol (IPA) be used instead of water for liver tissue sample preparation with the Triglyceride Quantification Colorimetric/Fluorometric Kit (MAK266)?

    1 answer

    1. Based on the guidelines, only water is an appropriate solvent for samples in this assay. Ethanol and isopropanol are not suitable solvents for this assay.

      Helpful?

  4. Is liver tissue extracted using RIPA buffer and without NP-40 compatible with the MAK266 Triglyceride Quantification Colorimetric/Fluorometric Kit?

    1 answer

    1. It is recommended to use NP-40 or similar non-ionic detergents instead of RIPA, due to the presence of harsh detergents in RIPA.

      Helpful?

3 Reviews This action will navigate to reviews.
1 of 3 reviewers received a sample product or took part in a promotion
0 out of 1 (0%) reviewers recommend this product

Reviews

Active Filters

  1. Pittsburgh, PA
    • Review 1
    • Votes 10
    1 out of 5 stars.

    Terrible kit!

    The protocol is not well-written. I looked for several literatures who used this kit and found inconsistent data for control samples. Even the technical staffs of Sigma could not satisfy my questions and I strongly discourage other shoppers to use this.

    Helpful?

    1. Response from Merck KGaA:

      Thank you for reaching out and sharing your review with us. We are sorry to hear about your experience with this product and is not what we would expect. Our records show your case was resolved as there were no additional follow up questions. We have provided your feedback to our Product Management team for further review.

  2. 2 Ratings-only reviews