L7395

Lipoxidase from Glycine max (soybean)

Type I-B, lyophilized powder, ≥50,000 units/mg solid

• Synonim(y): 15-lipoxygenase, LOX A, Linoleate:oxygen oxidoreductase, Lipoxygenase
• Numer CAS: 9029-60-1
• Numer EC enzymu: 1.13.11.12 (BRENDA, IUBMB)
• Numer WE: 232-853-1
• Numer MDL: MFCD00131527
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• typ: Type I-B
• Poziom jakości: 200
• Formularz: lyophilized powder
• aktywność właściwa: ≥50,000 units/mg solid
• masa cząsteczkowa: 94 kDa
• Warunki transportu: wet ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Lipoxidases (LOXs), or lipoxygenases, are nonheme iron proteins. Lipoxygenases are widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 with respect to pH and optimum temperature, substrate specificity, and level of inhibition by different agents.[1]

Zastosowanie

Lipoxidase from Glycine max (soybean) has been used:

• to study the effects of 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol(TMG) on oxidation of lipoproteins[2]
• as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives[3]
• as a positive control in lipoxygenase inhibitory activity assay[4]

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Działania biochem./fizjol.

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Lipoxidases are deactivated by heat treatment near 100°C for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases.[5] These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals.[1]

Definicja jednostki

One unit will cause an increase in A₂₃₄ of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A₂₃₄ unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Postać fizyczna

Contains stabilizer and NaCl

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)