P1274

Poly-L-lysine hydrobromide

suitable for cell culture, Mol wt 70,000-150,000 by viscosity

• Synonim(y): Bromowodorek homopolimeru L-lizyny
• Numer CAS: 25988-63-0
• Numer MDL: MFCD00237230
• Kod UNSPSC: 12352209
• Identyfikator substancji w PubChem: 24898180
• NACRES: NA.26

Właściwości

• Nazwa produktu: Poly-L-lysine hydrobromide, mol wt 70,000-150,000 by viscosity
• Formularz: solid
• Poziom jakości: 300
• masa cząsteczkowa: 70,000-150,000 by viscosity
• metody: cell culture | mammalian: suitable
• kolor: white to off-white
• temp. przechowywania: −20°C
• ciąg SMILES: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• Klucz InChI: WBSCNDJQPKSPII-KKUMJFAQSA-N

Opis

Zastosowanie

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Poly-L-lysine hydrobromide is used for the following applications:

• Used for some experiments with REF-52 cells, for coating[1]
• For electrophysiological experiments, cells were seeded on poly-L-lysine hydrobromide coated or uncoated cover-slips[2][3]
• Cell culture of rat neurons[4]
• Indirect binding of monoclonal antibody via poly-llysine[5]

Działania biochem./fizjol.

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Komponenty

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Przestroga

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Uwaga dotycząca przygotowania

This product has a molecular weight of 70,000-150,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Komentarz do analizy

Molecular weight based on viscosity and is also assayed by MALLS.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)