Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Dokumenty

GERPN2236

ECL™ Prime Western Blotting Detection Reagent

Cytiva RPN2236

Synonim(y):

chemiluminescent detection reagent, Western blotting detection reagent

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(1)

About This Item

Kod UNSPSC:
41105332
NACRES:
NA.32

opakowanie

pkg of 1 kit

producent / nazwa handlowa

Cytiva RPN2236

Opis ogólny

Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies, contributing to low background and reducing consumption of reagents.

Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.

Zastosowanie

ECL™ Prime Western blotting detection reagent has been used to detect chemiluminescent signals during Western blotting.

Cechy i korzyści

  • Compatible with Rainbow Molecular Weight Markers and Amersham™ ECL DualVue™ Western Blotting Markers.
  • High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity.
  • Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection.
  • Optimized for use with Amersham Hybond-P® (PVDF) membranes and compatible with Amersham Hybond-ECL (nitrocellulose) membranes.
  • Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham Hyperfilm™.

Komponenty

The following components are included in the Amersham ECL Prime detection reagent kit.
  • RPN2236 Solution A: Luminol solution, 3 × 50 ml Solution B: Peroxide solution, 3 × 50 ml sufficient for 3000 cm2 membrane

Przechowywanie i stabilność

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Komentarz do analizy

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informacje prawne

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
ECL DualView is a trademark of Amersham
Hybond is a registered trademark of Cytiva
Hyperfilm is a trademark of Cytiva
This page may contain text that has been machine translated.

Kod klasy składowania

12 - Non Combustible Liquids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Registered report: Melanoma genome sequencing reveals frequent PREX2 mutations
Denise Chroscinski
eLife, 3 (2014)

Produkty

Methods Used to Detect Proteins and Nucleic Acids Bound to Membranes

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

Metody stosowane do wykrywania białek i kwasów nukleinowych związanych z membranami

Informacje ogólne i protokoły opisujące różne metody stosowane przez biologów molekularnych do wykrywania próbek białek lub kwasów nukleinowych związanych z błonami.

Troubleshooting Detection Methods for GST-tagged Proteins

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

Protokoły

Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej