G115000

DMT-dG(dmf) Phosphoramidite

≥99.0% (reversed phase HPLC)

• Synonim(y): DMT-dG(dmf) Amidite, N-[(dimethylamino)methylene]-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxyguanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], N2-Dimethylformamidine-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite]
• Wzór empiryczny (zapis Hilla): C₄₃H₅₃N₈O₇P
• Numer CAS: 330628-04-1
• Masa cząsteczkowa: 824.90
• Numer MDL: MFCD00797420
• Kod UNSPSC: 12352200
• Identyfikator substancji w PubChem: 329799820
• NACRES: NA.51

Właściwości

• typ: for DNA synthesis
• Poziom jakości: 200
• linia produktu: Proligo Reagents
• Próba: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• Postać: powder
• masa cząsteczkowa: 824.90 g/mol
• metody: oligo synthesis: suitable
• kolor: white to off-white
• λ: conforms (UV/VIS Identity)
• profil nukleozydowy: base: deoxyguanosine; base protecting group: DMF; 2' protecting group: none; 5' protecting group: DMT; deprotection: fast
• temp. przechowywania: -10 to -25°C
• ciąg SMILES: COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(\N=C/N(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6
• InChI: 1S/C43H53N8O7P/c1-29(2)51(30(3)4)59(56-24-12-23-44)58-36-25-38(50-28-45-39-40(50)47-42(48-41(39)52)46-27-49(5)6)57-37(36)26-55-43(31-13-10-9-11-14-31,32-15-19-34(53-7)20-16-32)33-17-21-35(54-8)22-18-33/h9-11,13-22,27-30,36-38H,12,24-26H2,1-8H3,(H,47,48,52)/b46-27-/t36-,37+,38+,59?/m0/s1
• Klucz InChI: YRQAXTCBMPFGAN-UJASEYITSA-N

Opis

Opis ogólny

DMT-dG(dmf) Phosphoramidite belongs to fast deprotection phosphoramidites where the deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. Changing the dG protecting group to the dimethylformamidine (dmf) baseprotecting group enables the rapid synthesis of high-purity, high-yield oligonucleotide, thus increasing the efficiency of high-throughput production.

Cechy i korzyści

Key Features of dG(dmf):

• dG(dmf) is deprotected faster than the conventional dG(ib): the deprotection time in concentrated ammonia is reduced to 2 hours at 55 °C or 1 hour at 65 °C
• The dG(dmf)-monomer is especially suitable for G-rich sequences: incomplete deprotection is greatly reduced in comparison with the conventional dG(ib)-monomer
• dG(dmf)-phosphoramidite is as stable in solution as the standard dA(bz)-, dC(bz)- and dT-phosphoramidites
• dG(dmf)-phosphoramidite can directly substitute for dG(ib)-phosphoramidite
• No change is required in the reagents commonly used for DNA synthesis (Except a low concentration iodine oxidizer i.e., 0.02 M in iodine, should be employed)

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable