The Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of adenosine 5’-triphosphate (ATP) in samples containing 2x10–12 to 8x10–5 moles/liter. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin.
Zastosowanie
Adenosine 5′-triphosphate (ATP) assay mix has also been used to measure the level of cellular ATP in ATP viability assay.[1][2][3]
The ATP assay kit has been used to measure the level of cellular ATP present in isolated mitochondrial fractions from L. donovani promastigotes. [4]
Komponenty
Contains luciferase, luciferin, MgSO4, EDTA, DTT, BSA, and Tricine buffer.
Rekonstytucja
Reconstitute with 5 ml of sterile water.
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