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MAK135

Sigma-Aldrich

ADP/ATP Ratio Assay Kit

sufficient for 100 tests (bioluminescent)

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1 KIT
1770,00 zł

1770,00 zł

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1 KIT
1770,00 zł

About This Item

Kod UNSPSC:
12161503
NACRES:
NA.84

1770,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Poproś o zamówienie zbiorcze

zastosowanie

sufficient for 100 tests (bioluminescent)

Zastosowanie

pharmaceutical

metoda wykrywania

chemiluminescent

powiązane choroby

cancer

temp. przechowywania

−20°C

Powiązane kategorie

Opis ogólny

Changes in the ADP/ATP ratio have been used to differentiate modes of cell death and viability. Increased levels of ATP and decreased levels of ADP signify proliferating cells. Conversely, decreased levels of ATP and increased levels of ADP represent apoptotic or necrotic cells where the decrease in ATP and increase in ADP are much more pronounced in necrosis versus apoptosis.

Zastosowanie


  • LOC554202 przyczynia się do progresji chordoma poprzez sponging miR-377-3p i regulację w górę SMAD3: W niniejszym artykule zbadano mechanizmy molekularne, za pomocą których LOC554202 ułatwia progresję chordoma poprzez regulację miR-377-3p i SMAD3. Zestaw ADP/ATP Ratio Assay został wykorzystany do oceny metabolicznego wpływu tych zmian molekularnych na żywotność i proliferację komórek (Xu et al., 2023).

  • FOXG1 poprawia funkcję mitochondriówi promuje progresję raka jamy nosowo-gardłowej: W niniejszym badaniu zbadano, w jaki sposób FOXG1 poprawia funkcję mitochondriów, promując w ten sposób progresję raka jamy nosowo-gardłowej. Zestaw ADP/ATP Ratio Assay został wykorzystany do pomiaru funkcji mitochondriów i zmian metabolizmu energetycznego, zapewniając wgląd w profil bioenergetyczny komórek nowotworowych (Xi et al., 2021).

Cechy i korzyści

Compatible with high-throughput handling systems.

Przydatność

Suitable for the detection of apoptosis and necrosis in cells and for the studying the effects of compounds on cellular proliferation.

Zasada

The ADP/ATP Ratio Assay kit provides a simple and direct procedure for measuring ADP and ATP levels in cells for the screening of apoptosis, necrosis, and cell proliferation. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of the intracellular ATP concentration.

Luciferase
ATP + D-Luciferin + O2 ----------> oxyluciferin + AMP + PPi + CO2 + light

In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample.
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produkt powiązany

Numer produktu
Opis
Cennik

06693

Timestrip ™ -20 °C

Piktogramy

Exclamation mark
GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H317,H412

Zwroty wskazujące środki ostrożności

P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313

Klasyfikacja zagrożeń

Aquatic Chronic 3 - Skin Sens. 1

Kod klasy składowania

10 - Combustible liquids

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
135CF01A20
135CF01A20
135CE11A26
135CE06A24
135CE03A19

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Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Sangiliyandi Gurunathan et al.
Nanomaterials (Basel, Switzerland), 8(6) (2018-06-06)
The unique properties of gold nanoparticles (AuNPs) have attracted much interest for a range of applications, including biomedical applications in the cosmetic industry. The current study assessed the anti-oxidative effect of AuNPs against retinoic acid (RA)-induced loss of cell viability;
Nidal Zeineh et al.
Cells, 8(7) (2019-07-13)
The 18 kDa translocator protein (TSPO) is an initiator of the mitochondrial apoptosis cascade. Cigarette smoke (CS) exposure provokes alterations in TSPO expression as well as upregulation of its related functions such as mitochondrial membrane potential (ΔψM) and reactive oxygen
Aline G Cozer et al.
Lipids, 51(11), 1303-1307 (2016-10-25)
The present work assesses in vitro the role of human Stanniocalcin 1 (hSTC-1) in glucose metabolism in white retroperitoneal adipose tissue (WRAT) from fed rat. In the fed state, hSTC1 increases the incorporation of
Yixun Su et al.
Frontiers in cell and developmental biology, 8, 362-362 (2020-06-09)
The proliferation and differentiation of neural progenitor lay the foundation for brain development. In neural progenitors, activation of Signal Transducer and Activator of Transcription 3 (STAT3) has been found to promote proliferation and astrocytogenesis while suppressing neurogenesis. However, our study
Sangiliyandi Gurunathan et al.
Nanomaterials (Basel, Switzerland), 9(7) (2019-07-05)
Graphene, a two-dimensional carbon sheet with single-atom thickness, shows immense promise in several nanoscientific and nanotechnological applications, including in sensors, catalysis, and biomedicine. Although several studies have shown the cytotoxicity of graphene oxide in different cell types, there are no
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Questions

1–6 of 6 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. What is the appropriate concentration range for the assay? Can I use it to test the ADP/ATP ratio in buffer (~ 0.5 - 5 mM ATP and ADP)?

    1 answer

    1. The recommended sample volume is 10 microliters. The higher concentration of 5 mM that was mentioned corresponds to 50 nanomoles. This would be near the upper limit of the assay.
      Bioluminescent ATP assays are very sensitive and accurate over a broad range of the amount of ATP; nanomole and even picomole amounts of ATP can be determined. So, a 5 millimolar sample could be diluted 1000X to 5 micromolar and still give good results, and likely better results than the 5 mM sample.

      Helpful?

  4. Can I please see the product insert and instruction for use for product codeMAK135 ADP/ATP Ratio Assay kit

    1 answer

    1. Here is a link to the product protocol. It can also be found in the 'Documentation' section of the product page: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/135/374/mak135bul.pdf

      Helpful?

  5. Can the cells be stored in a freezer for future use with the MAK135-1KT ADP/ATP Ratio Assay Kit? Should the same approach recommended for tissue testing, involving snap freezing the tissue in liquid nitrogen and deproteinizing it to inactivate residual ATPases, be followed for testing cells?

    1 answer

    1. The approach for cells is similar to that for tissue samples, but with some differences. It is suggested to liquid nitrogen snap freeze the cells in DMSO to prevent cell lysis. Since these are cells, a deproteination step is not necessary. After thawing, the cells should be washed to remove the DMSO and replaced with PBS. Adding a phosphatase inhibitor such as sodium orthovanadate to the PBS can help limit undesired ATPase activity. It is advisable to resuspend the cells in an amount of PBS that allows them to dispense 10^3-10^4 cells in 10 uL sample volumes, as per the ELDT protocol. Quick work is advised, especially after thawing, to initiate the assay, as snap freezing and thawing the cells can stress them. It's preferable to assay freshly harvested cells over snap freezing, as the kit has only been tested on fresh cells.

      Helpful?

  6. How should this item be used on tissue samples? What is the preparation process and what type of buffer should be used?

    1 answer

    1. Given the relatively unstable nature of ATP, if immediate processing of the tissue and running the assay is not feasible after harvesting the tissue, it is advisable to snap freeze the tissue in liquid nitrogen. Prior to utilizing the tissue in the assay, it is recommended to deproteinate the tissue to deactivate any residual ATPases. This process involves initially homogenizing the tissue on ice in PBS + 1 mM EDTA + 0.5% Triton X100, deproteinating samples with TCA, then neutralizing to pH 7 with KOH. Following this, the sample should be centrifuged and the clear supernatant utilized for the assay. An alternative method involves using a 10 kDa spin column for the lysate.

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