FD20

Fluorescein isothiocyanate–dextran

average mol wt 20,000

• Synonim(y): FITC–Dextran
• Numer CAS: 60842-46-8
• Numer MDL: MFCD00131092
• Kod UNSPSC: 12352201
• NACRES: NA.25

Właściwości

• pochodzenie biologiczne: bacterial (Leuconostoc mesenteroides)
• białko sprzężone: FITC conjugate
• Postać: powder
• masa cząsteczkowa: average mol wt 20,000
• zakres etykietowania: 0.001-0.020 mol FITC per mol glucose
• kolor: light yellow to dark orange
• rozpuszczalność: water: 25 mg/mL
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Zastosowanie

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.

FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. Fluorescein-dextran, 20kDa, may be used to study cell processes such as endocytosis and osmoporation. FITC-dextran-20 may be used to study endothelial or epithelial monolayer porosity, apical-basolateral movements within cell layers and cell junction permeability. FITC-dextran-20 may be used as a model compound to study drug release from structures such as hydrogens and collagen minirods.

Opakowanie

Bottomless glass bottle. Contents are inside inserted fused cone.

Uwaga dotycząca przygotowania

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Inne uwagi

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H315,H319,H335
• Zwroty wskazujące środki ostrożności: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)