F8424

Fibroblast Growth Factor-4 human

≥95% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonim(y): hFGF-4, FGF-4, K-FGF
• Numer MDL: MFCD00675383
• Kod UNSPSC: 12352202
• NACRES: NA.77

Właściwości

• Nazwa produktu: Fibroblast Growth Factor-4 human, FGF-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• pochodzenie biologiczne: human
• Poziom jakości: 200
• rekombinowane: expressed in E. coli
• Próba: ≥95% (SDS-PAGE and HPLC)
• Formularz: lyophilized powder
• siła działania: ≤10.0 ng/mL
• jakość: endotoxin tested
• masa cząsteczkowa: 19 kDa
• opakowanie: pkg of 25 and 100 μg
• warunki przechowywania: avoid repeated freeze/thaw cycles
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: <1 EU/μg
• numer dostępu UniProt: P08620
• temp. przechowywania: −20°C
• informacje o genach: human ... FGF4(2249) , FGF4(2249)

Opis

Opis ogólny

Fibroblast Growth Factor-4 (FGF4) is a 19 kDa protein, belonging to the FGF family of proteins.[1] Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface.[2] There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34 kDa and they possess a conserved sequence of 120 amino acids.[3] The FGF4 gene is mapped to human chromosome 11q13.3.[4]

Zastosowanie

Fibroblast Growth Factor-4 human has been used:

• To maintain trophectoderm stem cells.[5]
• In neurogenic differentiation.[6]
• In hepatocyte differentiation.[7]

Działania biochem./fizjol.

Fibroblast growth factor-4 (FGF-4) is a 19 kDa protein identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development.

Fibroblast growth factor-4 (FGF-4) is identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development. FGF-4 participates in cell differentiation and cell migration.[1]

Postać fizyczna

Lyophilized from a 0.2 μm filtered buffer solution containing a carrier protein.

Komentarz do analizy

The biological activity is measured by the dose-dependent stimulation of thymidine uptake using BaF3 cells expressing FGF receptors.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)