F5392

Fibroblast Growth Factor-Basic from bovine pituitary

Name: Fibroblast Growth Factor-Basic from bovine pituitary
Brand: SIGMA
Price: 4010 PLN

~90% (SDS-PAGE and N-terminal analysis), lyophilized powder, suitable for cell culture

Synonim(y):

FGF2, bFGF

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1 μG

4010,00 zł

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Informacje o tej pozycji

Numer CAS:

106096-93-9

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00081382

Biological source:

bovine pituitary glands

Assay:

~90% (SDS-PAGE and N-terminal analysis)

Form:

lyophilized powder

Mol wt:

16.4 kDa

Impurities:

<0.5 EU/vial

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Nazwa produktu

Fibroblast Growth Factor-Basic from bovine pituitary, FGF-Basic, suitable for cell culture

biological source

bovine pituitary glands

assay

~90% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

potency

0.03-3.0 ng/mL ED₅₀/EC₅₀

quality

endotoxin tested

mol wt

16.4 kDa

packaging

pkg of 1 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<0.5 EU/vial

color

white

UniProt accession no.

P03969

storage temp.

−20°C

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Ta pozycja	F3685	F3133	F5542
Sigma-Aldrich F5392 Fibroblast Growth Factor-Basic from bovine pituitary	Sigma-Aldrich F3685 Fibroblast Growth Factor-Basic	Sigma-Aldrich F3133 Fibroblast Growth Factor from bovine pituitary	Sigma-Aldrich F5542 Fibroblast Growth Factor-Acidic human
biological source bovine pituitary glands	biological source human	biological source bovine pituitary glands	biological source human
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
assay ~90% (SDS-PAGE and N-terminal analysis)	assay ≥97% (SDS-PAGE)	assay -	assay ≥97% (SDS-PAGE)
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
impurities <0.5 EU/vial	impurities -	impurities endotoxin, tested	impurities ≤1.0 EU/mg (LAL test)

General description

The fibroblast growth factor-2 (FGF2) gene is predominantly expressed in uterus, cornea and vascular endothelial cells. The FGF2 gene is mapped to cattle chromosome 17.

Application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor-Basic from bovine pituitary has been used:

In animal and human cell culture
In neuronal induction of adipose and bone marrow-derived rat mesenchymal stem cells
In the generation of directly reprogramming fibroblast
In human glioma cell culture

Biochem/physiol Actions

Fibroblast growth factor-2 (FGF2) stimulates cell proliferation, differentiation and migration. It is also known to participate in angiogenesis and carcinogenesis. During the estrous cycle, FGF2 expression is found to be maximum in porcine conceptuses and endometrium. Upregulation of this gene is also observed in pregnancy during peri-implantation. FGF2 is involved in embryogenesis, tissue repair and other metabolic pathways.

Physical form

Lyophilized from a 0.2 μm filtered solution in sodium phosphate and sodium chloride containing 0.1 mg bovine serum albumin

Preparation Note

Purified glands by a modification of the method of Gospodarowicz, et al.[1]

Analysis Note

The proliferative activity is tested in culture using fetal bovine heart endothelial cells.

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Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: comparison of neural differentiation of rat AdMSCs and BMSCs

Capkin M, et al.

Biomedical Materials (Bristol, England), 7(4), 045013-045013 (2012)

Single nucleotide polymorphism of FGF2 gene in Iranian Holstein proven bulls

Salehi A, et al.

Molecular Biology Research Communications, 4(1), 57-57 (2015)

3D Endothelial Cell Migration.

Kathryn A Jacobs et al.

Methods in molecular biology (Clifton, N.J.), 1749, 51-58 (2018-03-12)

Endothelial cells have the capacity to shift between states of quiescence and angiogenesis. The early stage of angiogenesis, sprouting, occurs with the synchronized activities of tip cells, which lead the migration of the sprout, and stalk cells, which elongate this

Stimulatory effects of fibroblast growth factor 2 on proliferation and migration of uterine luminal epithelial cells during early pregnancy

Lim W, et al.

Biology of Reproduction, 96(1), 185-198 (2016)

Sensitivity of biochemical test in comparison with other methods for the detection of mycoplasma contamination in human and animal cell lines stored in the National Cell Bank of Iran

Kazemiha VM, et al.

Cytotechnology, 66(5), 861-873 (2014)

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