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Merck

F0291

Sigma-Aldrich

Fibroblast Growth Factor-Basic, human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonim(y):

Fibroblast Growth Factor-Basic human, FGF2, bFGF, hBFGF

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About This Item

Numer CAS:
Numer MDL:
Kod UNSPSC:
12352202
NACRES:
NA.77

Nazwa produktu

hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture

pochodzenie biologiczne

human

Poziom jakości

rekombinowane

expressed in E. coli

Próba

≥97% (SDS-PAGE)

Formularz

lyophilized powder

siła działania

≤-0.6 ng/mL

masa cząsteczkowa

16.0 kDa

opakowanie

pkg of 4X25 μg
pkg of 25 μg

warunki przechowywania

avoid repeated freeze/thaw cycles

metody

cell culture | mammalian: suitable

zanieczyszczenia

≤1.00 EU/μg

kolor

white to faint yellow cast

rozpuszczalność

water: soluble 0.025 mg, clear, colorless to faintly yellow

numer dostępu UniProt

temp. przechowywania

−20°C

informacje o genach

human ... FGF2 (2257)

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Opis ogólny

Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.

Zastosowanie

Fibroblast growth factor-basic (FGF-Basic) is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines, such as mesenchymal stem (stroma) cells (MSC).

Postać fizyczna

Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.

Komentarz do analizy

The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

R Beklem Bostancioglu et al.
Colloids and surfaces. B, Biointerfaces, 155, 415-428 (2017-05-02)
Accelerated Mesenchymal Stem Cells (MSCs) condensation and robust MSC-matrix and MSC-MSC interactions on nano-surfaces may provide critical factors contributing to such events, likely through the orchestrated signal cascades and cellular events modulated by the extracellular matrix. In this study, human
C Xu et al.
Nature biotechnology, 19(10), 971-974 (2001-10-03)
Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least
Chunhui Xu et al.
Stem cells (Dayton, Ohio), 23(3), 315-323 (2005-03-08)
Previous studies have shown that prolonged propagation of undifferentiated human embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders (MEF-CM) as well as matrix components. Because hESCs express growth factor receptors, including those for basic fibroblast growth factor
Structural characterization and biological functions of fibroblast growth factor.
D Gospodarowicz et al.
Endocrine reviews, 8(2), 95-114 (1987-05-01)
Brad Davidson et al.
Genes & development, 20(19), 2728-2738 (2006-10-04)
Comprehensive gene networks in Ciona intestinalis embryos provide a foundation for characterizing complex developmental processes, such as the initial phases of chordate heart development. The basic helix-loop-helix regulatory gene Ci-Mesp is required for activation of cardiac transcription factors. Evidence is

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