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F4165
Ficin from fig tree latex
powder, ≥0.1 unit/mg solid
Synonim(y):
Debricin, Ficain, higueroxyl delabarre
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 1000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 486,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-599-1
MDL number:
Specific activity:
≥0.1 unit/mg solid
Biological source:
fig tree (latex)
486,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
fig tree (latex)
Quality Level
form
powder
specific activity
≥0.1 unit/mg solid
mol wt
23.8 kDa
storage temp.
−20°C
General description
Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0
pI: 9.0
Biochem/physiol Actions
Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.
Other Notes
One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).
The enzyme is soluble in 1 M potassium phosphate buffer, pH 7.0 (0.25 mg/ml), yielding a clear solution.
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Ta pozycja | |||
|---|---|---|---|
| biological source fig tree (latex) | biological source fig tree (latex) | biological source fig tree (latex) | biological source - |
| specific activity ≥0.1 unit/mg solid | specific activity ≥1.0 units/mg protein | specific activity ≥1.0 units/mg protein (biuret) | specific activity 1.5-10 units/mg solid |
| form powder | form lyophilized powder | form saline suspension | form crude powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt 23.8 kDa | mol wt 23.8 kDa | mol wt 23.8 kDa | mol wt 23.4 kDa |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Klasa składowania
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Protokoły
This procedure may be used for all Ficin products.
Yufang Yang et al.
Scientific reports, 7, 43141-43141 (2017-02-23)
Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for
E Saitoh et al.
Archives of biochemistry and biophysics, 352(2), 199-206 (1998-05-20)
Two variants of cystatin SA encoded by two alleles at the CST2 locus of the type 2 human cystatin gene family were expressed in Escherichia coli. One, termed cystatin SA1, is identical to cystatin SA [S. Isemura, E. Saitoh, and
Eiichi Saitoh et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 141(1), 103-109 (2005-04-12)
A novel cysteine protease inhibitor (Eel-CPI-1) was isolated from the epidermis of the eel. Eel-CPI-1 was shown to bind strongly to both lactose- and carboxymethylated papain-affinity gels. Its molecular mass under reducing condition was determined to be 18 kDa by
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| F4165-1KU | 04061833617564 |