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A2404

Sigma-Aldrich

Alcohol Oxidase solution from Pichia pastoris

buffered aqueous solution, 10-40 units/mg protein (biuret)

Synonim(y):

Alcohol:oxygen oxidoreductase

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About This Item

Numer CAS:
9073-63-6
Numer EC enzymu:
1.1.3.13 (BRENDA, IUBMB)
Numer MDL:
MFCD00130452
Kod UNSPSC:
12352204
NACRES:
NA.54

Postać

buffered aqueous solution

aktywność właściwa

10-40 units/mg protein (biuret)

Warunki transportu

dry ice

temp. przechowywania

−20°C

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Opis ogólny

Alcohol oxidase (AOX) is a homo-octamer, which has a molecular weight of 600-kDa. It has eight flavin adenine dinucleotide (FAD) cofactors. It belongs to the glucose-methanol-choline (GMC) oxidoreductase family.

Zastosowanie

Alcohol Oxidase may be used to study protein translocation into peroxisomes. Product A2404 is from Pichia pastoris. It has been used for the bacterial expression and immunological verification of Hv-p68 cDNA clones .
Alcohol Oxidase solution from Pichia pastoris has been used in cotton pectin methylesterase (PME) enzyme assay. It has also been used to immobilize onto polyaniline (PANI) film as a visual biosensor in dip-stick format for the determination of ethanol in fermented beverage samples.

Działania biochem./fizjol.

Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It has the highest affinity for methanol. Alcohol oxidase is involved in the metabolism of methanol, which is important for yeasts that utilize methanol as their sole energy source. Alcohol oxidase is primarily found in the peroxisome but may be present in the cytoplasm as well.

Definicja jednostki

One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.

Postać fizyczna

Solution in 30% sucrose with 0.1 M phosphate buffer at pH 8.0
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A I Soldevila et al.
The Journal of biological chemistry, 276(7), 4652-4661 (2000-11-01)
We have cloned and sequenced a novel alcohol oxidase (Hv-p68) from the filamentous fungus Helminthosporium (Cochliobolus) victoriae that copurifies with mycoviral double-stranded RNAs. Sequence analysis revealed that Hv-p68 belongs to the large family of FAD-dependent glucose methanol choline oxidoreductases and
A simple visual ethanol biosensor based on alcohol oxidase immobilized onto polyaniline film for halal verification of fermented beverage samples
Kuswandi B, et al.
Sensors, 14(2), 2135-2149 (2014)
Structure of alcohol oxidase from Pichia pastoris by cryo-electron microscopy
Vonck J, et al.
PLoS ONE, 11(7), e0159476-e0159476 (2016)
Christina Dorado et al.
Biology, 10(12) (2021-12-25)
Florida citrus production has declined 75% due to Huanglongbing (HLB), a disease caused by the pathogenic bacterium Candidatus Liberibacter asiaticus (CLas). Methods to combat CLas are costly and only partially effective. The cross-compatible species Poncirus trifoliata and some of its
Xuan Wang et al.
Journal of experimental botany, 71(9), 2629-2640 (2020-02-02)
De-esterification of homogalacturonan (HG) is thought to stiffen pectin gels and primary cell walls by increasing calcium cross-linking between HG chains. Contrary to this idea, recent studies found that HG de-esterification correlated with reduced stiffness of living tissues, measured by
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Protokoły

Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)

To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.

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