Prepare stock solutions in 0.1 M Tris-HCl, pH 7.0. While no specific concentration was provided, calculations based on the assay procedure suggest that stock solutions should be in the range of 0.5 - 1.0 mg/ml protein. This aligns with the general recommendation for proteins of a minimum of 1 mg protein/ml. The stock solution can be stored as aliquots at -20°C and should be reasonably stable for several months.
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P4234
Pyranose Oxidase from Coriolus sp.
recombinant, expressed in E. coli, ≥2.7 units/mg solid
Synonim(y):
Pyranose: Oxygen 2-Oxidoreductase
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
250 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1470,00 zł |
1000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 5200,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥2.7 units/mg solid
Recombinant:
expressed in E. coli
1470,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Pozwól nam pomócrecombinant
expressed in E. coli
Quality Segment
form
powder
specific activity
≥2.7 units/mg solid
shipped in
wet ice
storage temp.
−20°C
General description
Pyranose oxidase (P2O), a homotetrameric protein consists of a covalently bound flavin adenine dinucleotide (FAD). It is seen mostly among wood-degrading basidiomycetes.
Application
Pyranose Oxidase from Coriolus sp. has been used in the enzymatic oxidation of D -glucose (DG). It has also been used as a component in oxygen scavenging system (OSS) to increase the lifetime of the fluorophores.
Biochem/physiol Actions
Pyranose oxidase (P2O) can be used in clinical chemistry to determine 1,5-anhydro-d -glucitol marker, used for glycemic control in diabetes patients.
Pyranose oxidase (P2O) catalyzes the oxidation of aldopyranoses at position C-2 to yield the corresponding 2-ketoaldoses. The in vivo substrates of P2O are thought to be D-glucose, D-galactose, and D-xylose. They are oxidized to 2-keto-D-glucose (D-arabino-hexos-2-ulose, 2-dehydro-D-glucose), 2-keto-D-galactose (D-lyxo-hexos-2-ulose, 2-dehydro-D-galactose), and 2-keto-D-xylose (D-threopentos-2-ulose, 2-dehydro-D-xylose), respectively. Pyranose oxidase has significant activity with carbohydrates such as, L-sorbose, D-glucono-1,5-lactone, and D-allose. When pyranose oxidase catalyzes the oxidation of aldopyranoses, electrons are transferred to molecular oxygen which results in the formation of hydrogen peroxide[1].
Other Notes
Contains glutamate
One unit produces 1.0 μmol of hydrogen peroxide per minute at 37 °C, pH 7.0.
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Ta pozycja | |||
|---|---|---|---|
| description recombinant, expressed in E. coli, ≥2.7 units/mg solid | description lyophilized powder, ≥35 units/mg protein (biuret) | description recombinant, expressed in E. coli, lyophilized powder | description lyophilized powder, 40-80 units/mg solid, pH 8.1 |
| specific activity ≥2.7 units/mg solid | specific activity ≥35 units/mg protein (biuret) | specific activity ≥35 unit/mg solid | specific activity 40-80 units/mg solid, pH 8.1 |
| form powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| recombinant expressed in E. coli | recombinant - | recombinant expressed in E. coli | recombinant - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the solution stability for product P4234 after dilution?
1 answer-
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Do you have a protocol for the activity assay used to qualify pyranose oxidase?
Do you have a protocol for the activity assay used to qualify pyranose oxidase?
1 answer-
We do not have a pre-formulated protocol available at the moment. However, for reference, you may find the following publication informative. The publication, "Limited proteolysis of pyranose 2-oxidase results in a stable and active complex" in PeerJ Materials Science 2:e7 (https://doi.org/10.7717/peerj-matsci.7), served as the basis for this assay, utilizing the production of quinoneimine dye to directly reflect the turnover rate of pyranose oxidase.
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